# Quantitative and temporal analysis of autophagy: Differential Response to amino acid and glucose starvation

## TL;DR

The paper studies how cells respond to amino acid and glucose starvation by measuring autophagy levels over time in living cells.

## Contribution

It provides a quantitative dataset showing that amino acid starvation, not glucose starvation, triggers autophagy in U2OS cells.

## Key findings

- Amino acid starvation induces autophagy, while glucose starvation does not in U2OS cells.
- MTORC1-mediated ULK1 regulation is closely linked to amino acid levels.
- Cells rapidly return to low autophagy when nutrients are restored.

## Abstract

Autophagy is a highly conserved, intracellular recycling process by which cytoplasmic contents are degraded in the lysosome. This process occurs at a low level constitutively; however, it is induced robustly in response to stressors, in particular, starvation of critical nutrients such as amino acids and glucose. That said, the relative contribution of these inputs is ambiguous, and many starvation medias are poorly defined or devoid of multiple nutrients. Here, we set out to create a quantitative dataset of autophagy across multiple stages in single, living cells, measured under normal growth conditions and during nutrient starvation of amino acids or glucose. We found that autophagy is induced by starvation of amino acids, but not glucose, in U2OS cells, and that MTORC1-mediated ULK1 regulation and autophagy are tightly linked to amino acid levels. While autophagy is engaged immediately during amino acid starvation, a heightened response occurs during a period marked by transcriptional upregulation of autophagy genes during sustained starvation. Finally, we demonstrated that cells immediately return to their initial, low-autophagy state when nutrients are restored, highlighting the dynamic relationship between autophagy and environmental conditions.