# Soaking Up Success: Sponge-Assisted Nanoparticle Transfection

## TL;DR

A new sponge-assisted method enables efficient mRNA delivery to hard-to-transfect cells using much smaller doses than traditional methods.

## Contribution

A novel sponge-assisted transfection method that drastically reduces required mRNA doses while maintaining efficiency.

## Key findings

- The sponge-assisted method achieves transfection at nanogram-scale mRNA doses.
- It works robustly across difficult-to-transfect cell types.
- The method is applicable for both research and therapeutic purposes.

## Abstract

Current cell transfection systems face efficiency limitations, particularly for challenging cell types. We present a sponge-assisted transfection method enabling efficient mRNA transfection at nanogram-scale doses—a several orders of magnitude-reduction compared to the microgram quantities typically required by lipid-based and membrane-disruption methods. This approach achieves robust, dose-dependent transfection across difficult-to-transfect cell types, establishing broad applicability for both basic research and therapeutic use.