# Mechanistic insights in the role of trehalose transporter in metabolic homeostasis in response to dietary trehalose

## TL;DR

This study explores how the sugar transporter HaST46 in insects helps regulate trehalose levels in response to diet.

## Contribution

The study identifies HaST46 as a key transporter modulating trehalose homeostasis through diet-dependent regulation.

## Key findings

- HaST46 is localized in the posterior midgut and its expression changes with dietary trehalose.
- HaST46 preferentially transports trehalose over glucose in Sf9 cells.
- Dietary trehalose reduces endogenous trehalose synthesis gene expression.

## Abstract

Trehalose is the major sugar in insect hemolymph and plays a diverse role. Its level is regulated by the dynamics of biosynthesis and distribution by sugar transporters. The metabolic balance between trehalose synthesis and uptake remains poorly understood, despite its critical role in homeostasis. Here, we examined the role of the putative gut-specific sugar transporter, HaST46, in regulating trehalose levels in Helicoverpa armigera, a model Lepidopteran pest. Analysis of publicly available transcriptomics, proteomics data, and qRT-PCR study suggests that HaST46 showed localization in the posterior midgut and its expression alters in response to dietary contents. The liquid chromatography-mass spectrometry (LC-MS) analysis of HaST46 overexpressing Sf9 cells indicated that trehalose transport is preferred over glucose. HaST46 expression was found to be modulated tissue-specific manner in response to dietary trehalose availability. Furthermore, trehalose synthesis genes were found to be downregulated in the case of a 50 mM trehalose diet. This suggests that a potential increase in exogenous trehalose uptake may attenuate its endogenous synthesis. HaST46 overexpression and silencing lead to altered trehalose levels in the insect, while also having impact on trehalose metabolizing enzymes. Overall, our findings reveal the role of gut predominant sugar transporter, HaST46, in metabolic fine-tuning between exogenous trehalose uptake and endogenous synthesis.