# Effects of grape pomace on growth performance, serum biochemical indices, amino acid composition, and rumen microbial diversity in Dorper × Hu hybrid sheep

## TL;DR

Adding grape pomace to sheep feed improves their health and amino acid levels without harming growth or microbial diversity.

## Contribution

This study is the first to show grape pomace's benefits on sheep's antioxidant status and muscle amino acids while reducing harmful bacteria.

## Key findings

- Grape pomace did not negatively affect the growth performance of hybrid sheep.
- It increased serum total protein and antioxidant capacity, and improved muscle amino acid content.
- Grape pomace reduced the abundance of potentially harmful rumen bacteria like Treponema.

## Abstract

Grape pomace (GP), a byproduct of wine-making, is rich in nutrients and bioactive compounds. However, its effects on the health and rumen microbial diversity of fattening rams remain insufficiently studied. This study investigated the impact of dietary GP supplementation on Dorper × Hu hybrid sheep.

Eighteen male F1 Dorper× Hu hybrid sheep were randomly divided into three groups. They were fed complete pelleted diets containing 0% (control), 15%, or 20% GP for 60 days. Growth performance, serum biochemical indices, amino acid profiles in the longissimus dorsi muscle, and rumen bacterial composition were evaluated.

The key findings were: (1) GP inclusion did not negatively affect growth performance. (2) It significantly increased serum total protein levels and total antioxidant capacity, and elevated the content of essential amino acids in the muscle. (3) While overall rumen microbial diversity was not altered, GP supplementation specifically reduced the abundance of certain potentially harmful bacteria, such as Treponema.

The results demonstrate that incorporating GP into the diet can enhance antioxidant status and improve muscle amino acid profiles in Dorper × Hu hybrid sheep, without compromising production performance. The selective reduction in specific rumen bacteria suggests a modulatory effect. This study provides a theoretical foundation for the effective utilization of GP in complete pellet feeds for ruminants.