# Syntaxin 3B Mediates Light‐Dependent Interactions with STXBP1 and Arrestin 4: Distinct Roles in Rods and Cones

## TL;DR

This study reveals how a protein called Syntaxin 3B interacts differently in rod and cone photoreceptor cells in the retina, affecting their function and survival.

## Contribution

The study identifies a light-dependent interaction network involving STX3, STXBP1, and ARR4, and shows distinct roles for STX3 in rod and cone photoreceptors.

## Key findings

- Cone-specific STX3 knockout leads to early cone dysfunction and progressive rod impairment.
- STX3 interacts with STXBP1 in light and with ARR4 in darkness, forming a light-regulated complex.
- Cone-specific depletion of STXBP1 and ARR4 occurs in the STX3 knockout model.

## Abstract

Syntaxin 3 (STX3), a member of the soluble N‐ethylmaleimide‐sensitive factor attachment protein receptor (SNARE) family, plays a central role in vesicle fusion. Beyond its synaptic localization, STX3 is also detected in the photoreceptor inner segment, where its function remains poorly understood. It is shown that STX3 interacts with rhodopsin, peripherin 2, and the rod outer segment protein 1. In rod‐specific STX3 knockout retinas, these proteins are mislocalized, whereas cone opsins remain properly localized, suggesting a distinct STX3 function in cones. To further define its cone‐specific dysfunction, a cone‐specific STX3 knockout mouse is generated. This model exhibited early cone dysfunction followed by progressive rod impairment and photoreceptor degeneration. Cone degeneration correlated with early abnormalities in the connecting cilium. Specifically, a selective depletion of syntaxin binding protein 1 (STXBP1) and cone arrestin 4 is observed, a phenotype not seen when STX3 is specifically eliminated in rods. A light‐dependent complex comprising STX3, STXBP1, and arrestin 4 is further identified, with arrestin 4 preferentially associating with STX3 in the dark‐adapted retina and with STXBP1 in the light‐adapted retina. These findings reveal a cone‐specific, light‐regulated protein interaction network essential for cone function and survival, highlighting distinct and context‐dependent roles of STX3 in rods and cones.

The present study investigates a cone‐specific STX3 knockout model, displaying a complete loss of cone function, a later onset reduction in rod function, and photoreceptor cell death. A cone‐specific depletion of STXBP1 and ARR4 could be observed in this model. STXBP1 preferentially bound to STX3 in light‐adapted and ARR4 in the dark‐adapted retina.