# Inhibition of Nuclear factor kappa B ameliorates bone destruction and osteoclastogenesis in collagen-induced arthritis mice

## TL;DR

Blocking the NF-ϰB pathway reduces joint swelling and bone damage in mice with collagen-induced arthritis.

## Contribution

This study shows that inhibiting NF-ϰB reduces osteoclastogenesis and inflammatory bone destruction in CIA mice.

## Key findings

- NF-ϰB inhibition lowered paw edema and arthritis scores in CIA mice.
- Inhibiting NF-ϰB reduced mRNA levels of inflammatory markers and osteoclast-related genes.
- The treatment ameliorated bone destruction by suppressing osteoclastogenesis.

## Abstract

We aimed to investigate the effect of interleukin-1β (IL-1β)/nuclear factor ϰB (NF-ϰB) pathway on osteoclastogenesis and inflammatory bone destruction in a mouse model of collagen-induced arthritis (CIA).

DBA/1 mice were divided into CIA group, NF-ϱB inhibitor group and control group. The degree of paw edema was measured and the score of paw arthritis was assessed. mRNA levels of IL-1β, matrix metalloproteinase-1 (MMP-1), tumour necrosis factor-α (TNF-α) levels, anti-tartrate acid phosphatase (TRAP), matrix metalloproteinase-9 (MMP-9), tissue proteinase K (CtsK) and integrin β3 (β3-Integrin) in the joints of mice were determined.

The paw edema score at 18-30 d and the paw arthritis score at 12-30 d in CIA group were higher than those in control group, and the paw edema score and paw arthritis score at 24-30 d in NF-kB inhibitor group were lower than those in CIA group. mRNA levels of IL-1β, MMP-1, TNF-α in serum and TRAP, MMP-9, CtsK and β3-Integrin in joint tissues in CIA mice were higher than in controls. NF-ϰB inhibitor treatment significantly decreased the above mRNA levels both in serum and joint tissues.

NF-ϰB pathway inhibition can ameliorate bone destruction in the foot joints of CIA mice, which may be related to the reduction of inflammatory bone destruction and osteoclastogenesis.