Anti-inflammatory and cancer chemopreventive potential of essential oils from some cultivated plants in Egypt

TL;DR

This study explores the anti-inflammatory and cancer chemopreventive effects of essential oils from four Egyptian plants, finding that Artemisia abrotanum is the most effective.

Contribution

The study provides new evidence on the anti-inflammatory and chemopreventive potential of essential oils from specific Egyptian plant species.

Findings

Artemisia abrotanum essential oil showed 96.6% inhibition of nitric oxide release, indicating strong anti-inflammatory activity.

Lavandula dentata and Artemisia abrotanum essential oils moderately induced the expression of the cancer chemopreventive marker NQO1.

Artemisia abrotanum essential oil significantly inhibited iNOS protein expression at 100 µg/ml.

Abstract

This study aimed to investigate the biological activity of essential oils (EOs) of Artemisia abrotanum, Lavandula dentata, Cymbopogon citratus and Laurus nobilis as anti-inflammatory and cancer chemopreventive activities. The anti-inflammatory activity was evaluated using lipopolysaccharides-induced nitric oxide (NO) inhibition on murine macrophage cells (RAW264.7) and cancer chemo-preventive influence was assessed in vitro utilizing Hepa1c1c7 murine carcinoma cells. The EO of A. abrotanum has the most potential activity toward inhibition of NO release, recording 96.6 ± 0.1%, as estimated by Greiss assay. Followed by EOs of L. dentata and L. nobilis with 63.6 ± 0.11 and 37.0 ± 0.23%, respectively. At the protein expression level, western blotting technique was used to evaluate the expression of iNOS. The EO of A. abrotanum at 100 µg/ml exhibited a very high impact on inhibiting iNOS expression, followed by EOs of L. dentata and L. nobilis. On the other hand, pre-screening concentration (100 µg/ml) revealed that the EOs of L. dentata and A. abrotanum have moderate potency to induce expression of chemo-preventive marker NQO1. The results revealed that the EO of A. abrotanum had strong anti-inflammatory activity. While the EOs of L. dentata and A. abrotanum have moderate potency to induce cancer chemoprevention.

The online version contains supplementary material available at 10.1038/s41598-026-35195-0.

Figures

Inhibition of LPS-induced NO in RAW264.7 cells. Indicated cells were cultured as monolayers and treated as mentioned in the Materials and Methods section. Data are means ± SEM (*n* = 2). * denotes significance level of *P* < 0.05 (one-way ANOVA and Dunnett’s test).

Effect of essential oils (100 µg/ml) on LPS-induced protein expression of iNOS in RAW264.7 cells. Indicated cells were cultured as monolayers and treated as mentioned in the Materials and Methods section. An uncropped image of all blots shown is provided in supplementary material as Figure S1.

Effect of EO of *A. abrotanum* in gradual concentration on LPS-induced protein expression of iNOS in RAW264.7 cells. Indicated cells were cultured as monolayers and treated as mentioned in the Materials and Methods section. An uncropped image of all blots shown is provided in supplementary material as Figure S2.

Up-regulation of NQO1 protein expression by essential oils (100 µg/ml) in Hepa1c1c7 cells. Indicated cells were cultured as monolayers and treated as mentioned in the Materials and Methods section. An uncropped image of all blots shown is provided in supplementary material as Figure S3.

Concentration-dependent regulation of NQO1 protein expression by essential oils in Hepa1c1c7 cells. Indicated cells were cultured as monolayers and treated as mentioned in the Materials and Methods section. An uncropped image of all blots shown is provided in supplementary material as Figure S4.

Concentration-dependent regulation of HO-1 protein expression by essential oils in Hepa1c1c7 cells. Indicated cells were cultured as monolayers and treated as mentioned in the Materials and Methods section. An uncropped image of all blots shown is provided in supplementary material as Figure S5.

Full text

Introduction

Inflammation is a natural, complicated and vital process for tissue defense and repair. It is a crucial innate immune response that safeguards the body against infection and injury by activating immune cells and molecular signals to the injured tissues. Acute inflammation has a preventive and self-limiting function. However chronic inflammation can be injurious and is linked to numerous disorders including cancer^1^. The inflammatory process results from cellular and vascular events induced by substances known as inflammatory mediators. They are vital molecules that coordinate the intricate processes of inflammation, balancing defense and tissue healing. Among these molecules are nitric oxide (NO), cytokines, chemokines, eicosanoids and vasoactive amines^2^.

Persistent activation of inflammatory pathways has been recognized as a critical driver in the multistep process of carcinogenesis. Plants are thus increasingly recognized for their potential in chemoprevention due to active phytochemicals that can simultaneously enhance cellular defense mechanisms and attenuate inflammation. Many studies have explained in detail the strong link between chronic inflammation and cancer development^3–6^. Chronic injury due to inflammation also causes alteration of signal transduction proteins, disruption of DNA integrity and the possible activation of pathways with carcinogenic potential^4^. Inflammation confers on the microenvironment a cancer-friendly atmosphere through activating various pro-inflammatory signaling molecules, and targeting inflammatory signaling thus remains one of the promising approaches for cancer prevention^6^.

Cancer chemoprevention involves the application of diverse modalities, both natural and synthetic, to inhibit, prevent or postpone cancer by obstructing tumor formation in its early stages or by decelerating its growth rate, thus deferring the malignant properties and behavior of tumors. This prevention includes interrupting or delaying multiple molecular pathways, among which is the nuclear factor E2-related factor 2 (Nrf2) pathway. When Nrf2 is activated in the Keap1/Nrf2 signaling pathway, it translocates to the nucleus and attaches to the antioxidant response elements (ARE) of target genes. Nrf2 regulates the expression of several enzymes associated with phase II metabolism and safeguards cells against oxidative damage. These enzymes include heme oxygenase 1 (HO-1), NADPH: quinone oxidoreductase 1 (NQO1) and γ-glutamylcysteine synthetase (γGCS). NQO1 generates reduced forms of tocopherols and ubiquinone, making them effective in neutralizing free radicals. HO-1 serves a crucial antioxidant function in preserving cellular homeostasis against ROS^7,8^.

There are a number of plant-based phytochemicals known to activate the transcription factor Nrf2, which controls the expression of NQO1. The expression of detoxification enzymes in response to pro-carcinogens is mediated through the Nrf2/Keap1 signaling pathway, considered one of the most important mechanisms by which the cell defends against carcinogenic stress^9,10^.

Natural products derived from bacteria, marine organisms and plants have been essential for the development of inflammation and cancer treatments due to their wide chemical diversity, unique structural characteristics and biological efficacy, which exhibit less toxicity. These products become great importance in the prevention and treatment of cancer, which induce apoptosis, inhibit cell invasion and metastasis, exhibit anti-inflammatory properties and enhance immune responses^11^.

Medicinal and aromatic plants have played a vital role in the healthcare practices of various civilizations throughout human history, providing natural treatments and serving as a fundamental resource for the development of current pharmaceuticals^12^. In the last few years, much attention has been given to the essential oils (EOs) and pharmacological activities of aromatic plants. Many studies suggest that the pharmacological activities of these plants are particularly related to the chemical composition of their EOs. Essential oils exhibit significant therapeutic potential, especially in cancer therapy. These oils have bioactive substances, chiefly terpenoids and phenylpropanoids, which demonstrate antioxidant, anti-inflammatory and antimicrobial capabilities, providing defense against several metabolic and infectious disorders^13,14^.

Among the promising species were Artemisia abrotanum L. “Asteraceae, commonly known as southernwood”, Lavandula dentata L. “Lamiaceae, commonly known as lavender”, Cymbopogon citratus (DC.) Stapf “Poaceae, commonly known as lemongrass” and Laurus nobilis L. “Lauraceae, commonly known as bay laurel”.

Previous studies on the extracts prepared from these species: “A. abrotanum, L. dentata, C. citratus and L. nobilis” demonstrated various biological activities including analgesic, antioxidant, anti-inflammatory, anti-proliferative, antidepressant, anti-diabetic, anti-asthma, hypolipidemic, hypoglycemic, anti-acetyl-cholinesterase, anti-nociceptive, antidiarrheal, antimalarial, immunosuppressant effect, apoptotic effect, antimicrobial and antiviral activities^15–34^. Furthermore, EOs distillated from these species showed different biological activities such as antioxidant, cytotoxic, antimicrobial, anticoagulant, analgesic and anti-diabetic, hypotension, vasorelaxation, anxiolytic, anticonvulsant, depressant, neuroprotective, anti-inflammatory, anticholinesterase activities and reduced the blood cholesterol level^35–53^.

According to our previous publication on the chemical composition of these essential oils^54^, the most prominent constituents of C. citratus were E-citral (36.7%) and Z-citral (32.2%), followed by β-myrcene (8.8%). Whereas, the major constituents of L. dentata were eucalyptol (31.2%), camphor (19.7%) and α-pinene (5.5%). On the other hand, the most prominent compounds of L. nobilis were eucalyptol (35.5%), α-terpinyl acetate (16.3%) and sabinene (10.6%). Also, A. abrotanum has artemisia ketone (31.8%), α-eudesmol (20.6%) and γ-eudesmol (5.1%) as the most prominent constituents.

In the present study, we investigated the biological efficacy of the essential oils of southernwood, lavender, lemongrass and bay laurel, with a focus on their anti-inflammatory activity and potential roles in cancer chemoprevention. A robust cell-based model of lipopolysaccharide (LPS)-induced inflammation in RAW264.7 macrophages was employed to assess anti-inflammatory activity. While chemopreventive potential was evaluated by measuring the induction of NQO1, a principal marker of Nrf2 activation, in the murine Hepa1c1c7 cell line.

Experimental

Chemicals and reagents

Dulbecco’s modified Eagle’s medium (DMEM), α-modified minimum essential medium Eagle (α-MEM), Trypsin-Versene (EDTA) Mix, Dulbecco’s Phosphate-Buffered Saline (DPBS), L-Glutamine and penicillin/streptomycin stock (Lonza Verviers SPRL, Belgium). Fetal Bovine Serum (FBS; SeraLab, UK); Lipopolysaccharide (LPS; Sigma-Aldrich, from Escherichia coli serotype O111:B4), Enzyme chemiluminescence (ECL; Thermofisher scientific, USA) and Tris-buffered saline with 0.1% Tween 20 (TBST).

Plant materials

The aerial parts of L. dentata, A. abrotanum and L. nobilis were collected from the experimental farm of the National Research Centre in December, while C. citratus was collected from Agricultural Research Station in Al-Qanatir Al-Khairiya in December. Plants kindly identified by Dr. Mohamed El-Gebally, former Researcher of Botany, National Research Centre, Dokki, Cairo, Egypt. The specimens are stored in the herbarium of National Research Centre under the voucher numbers; M277 for A. abrotanum, M278 for C. citratus, M279 for L. nobilis and M280 for L. dentata.

Extraction of EOs

One hundred grams of fresh aerial parts of collected plants were subjected to water distillation using Clevenger’s apparatus. The distillation persisted for 3 h from the onset of boiling. The volatile substances were collected, dehydrated using traces of anhydrous sodium sulfate, filtered and preserved at -20℃ until required^55^.

Cell culture

Murine macrophage (RAW264.7) and murine hepatoma (Hepa1c1c7) cells were purchased from ATCC^®^. RAW264.7 cells were cultured as monolayer in DMEM medium enriched with 10% (v/v) heat-inactivated FBS. Hepa1c1c7 cells were cultured as a monolayer in α-MEM medium enriched with 10% (v/v) heat and charcoal–inactivated FBS. Both media supplemented with 100 µg/ml streptomycin sulfate, 100 U/ml penicillin and 4 mM L-glutamine. The cells were incubated in a humidified CO_2_ incubator with 5% CO_2_ at 37℃.

Anti-inflammatory activity

A model for the inhibition of LPS-induced NO release in RAW264.7 was carried out as previously described^56,57^. Briefly, cells (1.5 × 10^6^ cells/well) were plated in 6-well plates and incubated for 24 h. On the following day, cells were treated with either 0.1% v/v DMSO (negative control, LPS^−^), 100 ng/ml LPS (LPS^+^) or LPS combined with samples (100 µg/ml), then incubation for 24 h. Indomethacin (250 µM) served as the positive control.

iNOS activity assay

The Griess assay^58^ was utilized to determine NO in culture supernatants after 24 h. Briefly, 100 µl of culture supernatant from each well were combined with an equal volume of Griess reagent, mixed at room temperature and absorbance was measured at 520 nm using a TriStar2 LB 942 microplate reader (Berthold Technologies, Bad Wildbad, Germany). The percentage of inhibition was calculated relative to the LPS only group (LPS^+^). The following formula was used to calculate the % inhibition of NO release:

\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\text{Inhibition of LPS} - \text{induced NO}\ (\%) = \frac{{\rm Ab} - {\rm As}}{{\rm Ab}} \times 100$$\end{document}

Whereas; Ab: Absorbance of LPS^+^. As: Absorbance of sample.

Preparation of cell lysate for the assessment of iNOS protein expression

Western blotting analysis was employed to assess the protein expression of the pro-inflammatory marker, inducible nitric oxide synthase (iNOS). After exposure for 24 h as mentioned above, cells were rinsed with ice-cold DPBS. Cells were subsequently scrapped in ice-cold homogenization buffer (25 mM Tris-Cl, pH 7.4, 250 mM sucrose) supplemented with protease/phosphatase inhibitor cocktail (Halt^®^, Thermo-fisher scientific, USA) and transported to appropriately labeled micro-centrifuge tubes. Cell suspensions were subsequently sonicated on ice for 5 s at 30% amplitude. Cell lysates were then centrifuged at 12,000 ×g for 5 min and the supernatants (cytosolic fractions) were collected. Total protein content was assessed using a NanoDrop spectrophotometer (Thermo Fisher Scientific Inc., Waltham, Massachusetts, USA). Samples were then stored at -80℃ freezer until assayed for protein expression utilizing western blotting technique.

Cancer chemopreventive activity

The induction of NAD(P)H: quinone oxidoreductase 1 (NQO1) and heme oxygenase 1 (HO-1) in Hepa1c1c7 cells was assessed^59^. Briefly, cells (3 × 10^5^ cells/ml) were plated in 6-well plates and incubated overnight to adhere and form semi-confluent monolayers. Monolayers were treated with either 0.1% v/v DMSO (negative control), samples or 4‵-bromoflavone (4‵-BF, 10 µM) served as positive control for 4 h (HO-1) or 48 h (NQO1). Subsequently, treatment media were discarded and monolayers were rinsed with ice-cold DPBS (2 ml/well). Cells were then scrapped in ice-cold lysis buffer (5 µM FAD, 250 mM sucrose and 25 mM Tris-Cl, pH 7.4) and transported to appropriately labeled micro-centrifuge tubes. Cell suspensions were subsequently sonicated on ice for 5 s at 30% amplitude. Cell lysates were then centrifuged at 12,000 ×g for 5 min and the supernatants (cytosolic fractions) were collected. Total protein content was assessed using a NanoDrop spectrophotometer (Thermo Fisher Scientific Inc., Waltham, Massachusetts, USA). Samples were then stored at -80℃ freezer until assayed for protein expression utilizing western blotting technique.

Western blotting technique

Proteins were resolved under denaturing conditions by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) on a 10% acrylamide/bisacrylamide gel and electrophoresed for 90 min at 110 volts using a Biorad Tetra Cell (Biorad, USA). Subsequently, the resolved proteins were transferred to nitrocellulose membrane (0.45 μm pore size) using a Biorad transfer module at 100 volts for 60 min. Following electro-blotting, blots were cut alongside the Mwt. ladder onto appropriate strip for each protein target. Membrane strips were then blocked for 1 h with 5% non-fat milk. Strips were then incubated at 4℃/overnight with corresponding primary antibodies against iNOS (1:1000, Biomatik, Canada), NQO1 (1:1000, Elabscience, USA), HO-1 (1:500, Thermo-fisher scientific, USA) or β-actin (1:2000, Thermo-fisher scientific, USA). Then, 3 × 5 min washes with TBST, the membranes were incubated with appropriate horseradish peroxidase-conjugated secondary antibodies for 1 h at room temperature, followed by 3 × 5 min washes with TBST. Membrane proteins were developed utilized ECL western blotting substrate for 5 min, subsequently imaging was conducted utilizing a UVP Biospectrum Imager (Analytik Jena, Germany).

A dose-response experiment was performed on samples demonstrated high protein expression utilizing concentrations of 6.25, 25 and 100 µg/ml.

Statistical analysis

Statistical analysis of the data was conducted utilizing one-way analysis of variance (ANOVA) and Dunnett’s multiple comparisons test via GraphPad Prism^®^ V6.0 software (GraphPad Inc., San Diego, USA). The results are expressed as mean ± standard error of the mean (SME). The statistical significance was determined at P < 0.05.

Results

Evaluation of anti-inflammatory potential

The innate immune system can be activated by lipopolysaccharide (LPS), which is known to cause inflammation^60^. In macrophages, LPS interacts with the Toll-like receptor 4 (TLR4), which leads to the synthesis of many pro-inflammatory mediators, involving nitric oxide (NO) which is released by the inducible nitric oxide synthase (iNOS) enzyme^61^.

In this study, we used the murine macrophage RAW264.7 model as a robust and inducible cell for the induction of inflammation by LPS, which is a type recognition molecule and a component of the cell wall of gram-negative bacteria^62^.

iNOS activity assay (Greiss assay)

The EOs were initially pre-screened in vitro at 100 µg/ml for their anti-inflammatory potential. As displayed in Fig. 1, the pre-screening revealed that the EO of A. abrotanum has the most potential activity for inhibition of NO release, recording 96.6 ± 0.1% inhibition of LPS-induced NO, as estimated by the Greiss assay. Whereas, both EOs of L. dentata and L. nobilis exhibited 63.6 ± 0.11 and 37.0 ± 0.23% inhibition of LPS-induced NO, respectively. Furthermore, the EO of C. citratus exhibited a strong cytotoxic effect against RAW264.7 cells.

In addition, indomethacin (250µM) was used as a positive drug and showed 54.1 ± 0.11% inhibition of LPS-induced NO.

Fig. 1. Inhibition of LPS-induced NO in RAW264.7 cells. Indicated cells were cultured as monolayers and treated as mentioned in the Materials and Methods section. Data are means ± SEM (n = 2). * denotes significance level of P < 0.05 (one-way ANOVA and Dunnett’s test).

Protein expression level

At the protein expression level, a western blotting technique was used to confirm the NO inhibition at the level of iNOS protein expression. The EO of A. abrotanum at 100 µg/ml exhibited a very high impact on the inhibition of iNOS expression, followed by EOs of L. dentata and L. nobilis as shown in Fig. 2.

An additional experiment was performed on the EO of A. Abrotanum, which showed the greatest ability to inhibit iNOS protein expression, with increasing concentrations (6.25, 25 and 100 µg/ml) and showed concentration-dependent inhibition of iNOS protein expression induced by LPS, as displayed in Fig. 3. The highest inhibitory effect of EO of A. Abrotanum at 100 µg/ml, followed by 25 and 6.25 µg/ml on iNOS protein expression.

Fig. 2. Effect of essential oils (100 µg/ml) on LPS-induced protein expression of iNOS in RAW264.7 cells. Indicated cells were cultured as monolayers and treated as mentioned in the Materials and Methods section. An uncropped image of all blots shown is provided in supplementary material as Figure S1.

Fig. 3. Effect of EO of A. abrotanum in gradual concentration on LPS-induced protein expression of iNOS in RAW264.7 cells. Indicated cells were cultured as monolayers and treated as mentioned in the Materials and Methods section. An uncropped image of all blots shown is provided in supplementary material as Figure S2.

Evaluation of cancer chemoprevention

NAD(P)H: quinone oxidoreductase 1 (NQO1) is an antioxidant enzyme that plays an important role in cancer chemoprevention. NQO1 preserves cells from oxidative stress by converting quinones into hydroquinones^63^. The nuclear factor‑erythroid factor 2‑related factor 2 (Nrf2) stimulates NQO1, heme oxygenase 1 (HO-1) expressions and other cytoprotective enzymes in reacting to cellular stress^8^.

Treatment of Hepa1c1c7 cells with a pre-screening concentration (100 µg/ml) revealed that the EOs of L. dentata and A. abrotanum have moderate potency to induce expression of chemo-preventive marker NQO1 as evaluated by western blotting technique, as illustrated in Fig. 4.

Whereas, the EOs of C. citratus and L. nobilis exhibited a strong cytotoxic effect against Hepa1c1c7 cells as observed under an inverted microscope.

Fig. 4. Up-regulation of NQO1 protein expression by essential oils (100 µg/ml) in Hepa1c1c7 cells. Indicated cells were cultured as monolayers and treated as mentioned in the Materials and Methods section. An uncropped image of all blots shown is provided in supplementary material as Figure S3.

An additional experiment was performed on the EOs of A. Abrotanum and L. dentata which showed a moderate ability to induce expression of NQO1, with increasing concentrations (12.5, 50 and 200 µg/ml). Also, a dose-response experiment was conducted to assay expression of HO-1 at 12.5, 50, 200 µg/ml. Different concentrations showed almost similar induction of the expression of chemo-preventive marker NQO1 protein as shown in Fig. 5. In addition, the induction of chemo-preventive marker HO-1 protein expression was increased at concentration-dependent, as shown by western blotting technique, and the maximum induction of HO-1 appeared at a concentration of 200 µg/ml, as illustrated in Fig. 6.

Essential oils have attracted considerable interest for their possible health advantages, especially concerning oxidative stress and inflammation. A significant area of investigation examines their impact on the Nrf2 signaling pathway, which is essential for regulating the body’s antioxidant response through the modulation of various cytoprotective genes, such as NQO1 and HO-1, with particular emphasis on their role in cancer chemoprevention.

Fig. 5. Concentration-dependent regulation of NQO1 protein expression by essential oils in Hepa1c1c7 cells. Indicated cells were cultured as monolayers and treated as mentioned in the Materials and Methods section. An uncropped image of all blots shown is provided in supplementary material as Figure S4.

Fig. 6. Concentration-dependent regulation of HO-1 protein expression by essential oils in Hepa1c1c7 cells. Indicated cells were cultured as monolayers and treated as mentioned in the Materials and Methods section. An uncropped image of all blots shown is provided in supplementary material as Figure S5.

Discussion

The two major transcription factors; one responsible for cellular defense against oxidative stress and the other against inflammation—are Nrf2 and NF-κB, respectively. Although they are closely interrelated, these two major cellular stress-response systems often operate as functional antagonists. Their balance plays an important role in inflammation, oxidative stress, and cancer prevention. Loss of Nrf2 function may enhance NF-κB activity through increased cytokine production, while NF-κB modulates the transcription and activity of Nrf2 with both positive and negative influences on the target gene expression^64^.

In the present study, the EO of A. abrotanum exhibited strong anti-inflammatory potential against LPS-stimulated production of NO and expression of iNOS in RAW264.7 macrophages. Whereas the EO of L. dentata showed mediated anti-inflammatory effect. In contrast, the EO of L. nobilis exhibited low activity. These differences in biological activity can most likely be explained by differences in the chemical composition of the respective essential oils.

As shown in our previous publication^54^, the major constituents of A. abrotanum were artemisia ketone, α-eudesmol and γ-eudesmol. On the other hand, the most prominent compounds of L. dentata were eucalyptol, camphor and α-pinene.

To date, no studies have showed the anti-inflammatory properties of Artemisia ketone. On the other hand, α-eudesmol has shown promising anti-inflammatory activity through various molecular mechanisms. In an in silico study, Mondal et al.^65^ stated that α-eudesmol showed high binding affinity for crucial mediators of inflammation like interleukin-1β–converting enzyme, thus justifying its potential as a bioactive anti-inflammatory agent.

Eucalyptol is an established anti-inflammatory compound for which extensive evidence exists at the cellular, animal and clinical levels. It exerts anti-inflammatory effects by suppressing LPS–induced pro-inflammatory cytokines, including NF-κB, TNF-α, IL-1β and IL-6. It further modulates the extracellular signal-regulated kinase (ERK) pathway and diminishes oxidative stress by regulating redox-sensitive signalling and radical-scavenging activity^66^. Also, camphor has context-dependent and dose-sensitive immune-pharmacological properties, exhibiting both anti-inflammatory and potentially pro-inflammatory actions. Experimental work by Silva-Filho et al.^67^ showed that orally administered camphor inhibited leukocyte recruitment and reduced edema formation at a dosage of 100–200 mg/kg.

To the best of our knowledge, there are no previous studies that showed the anti-inflammatory activity of EO of A. abrotanum. However, the anti-inflammatory activity of different species of the genus Аrtemisia were previously published by other investigators. The inhibitory effect of the EO of Аrtemisia fukudo on the production of NO and PGE2 induced by LPS was due to suppression of iNOS and COX-2 expression^68^. It also inhibits NF-κB activation by preventing the nuclear translocation of p65 and p50 proteins. In addition, it is a powerful inhibitor of the production of NO, TNF-α, PGE2 and interleukins (IL-6 and IL-1β) in RAW264.7 cells induced by LPS and produces these effects through interaction at the transcription level. Furthermore, the anti-inflammatory effect of the EO of Аrtemisia fukudo was associated with the inactivation of MAPKs and NF-κB caused by blocking the phosphorylation of IkB-α, ERK, JNK and p38^68^. The EO of Аrtemisia herba-alba showed the efficacy to decrease the production of NO in RAW264.7 cells from 100 to 18.59 ± 1.26% at 1.25 µl/ml and BV-2 cells from 100 to 32.93 ± 1.51 at 1.25 µl/ml^69^. Zhu et al.^70^ demonstrated that the pre-treatment of RAW264.7 cells with the EO of Аrtemisia annua inhibit the production of IL-6, NO and TNF-α with inhibition rates 55.55, 47.84 and 26.57%, respectively. Also, Trinh et al.^71^ exhibited that the treatment of LPS-stimulate RAW264.7 cells with the EO of Аrtemisia vulgaris led to reduce NO generation by 9.48–58.43% at a gradual concentration from 12.5 to 100 µg/ml. In addition, the EO of Аrtemisia vulgaris decreased TNF-α by 11.6–55.6%.

The EO of Lavandula viridis exhibited significant inhibition of NO production by LPS in RAW264.7 cells with 96.15% inhibition at 0.64 µl/ml. In addition, pre-treatment of cells with the EO significantly suppressed iNOS and COX-2 protein expression by 97.40 and 53.9%, respectively, without any effect on the COX-1 level. It also caused significant inhibition of IκB-α phosphorylation and reduced the mRNA levels of iNOS and pro-inflammatory (IL-1β and IL-6)^72^. Wei et al.^73^ demonstrated that the EO of Lavandula angustifolia inhibited NO and ROS production in LPS-stimulated HaCaT (spontaneously immortalized human keratinocyte cells) and RAW264.7 cells, and suppressed gene expression of TNF-α, IL-6 and IL-1β at the protein and mRNA levels. 0.1% of EO reduced the production of ROS with 74.09 and 46.75%, respectively. It also had the suppression of iNOS protein expression at 0.01% of EO with inhibition rates of 49.49 and 32.49%, respectively. In addition, the EO at 0.001, 0.01 and 0.1% exhibited inhibition of p38 and JNK phosphorylation with inhibition rates ranging from 20.15 to 43.46%. In LPS-stimulated HaCaT cells, 0.1% of EO suppressed IκBα and p50 phosphorylation, with inhibition rates reaching to 65.10 and 60.44% but promoted p65 phosphorylation. On the other hand, in LPS-stimulated RAW264.7 cells, the EO at 0.001, 0.01 and 0.1% showed inhibitory effects against phosphorylation of IκBα, p65 and p50 with inhibition rates ranging from 10 to 50%.

The EO of L. nobilis significantly suppressed the progressive increase in inflammation in formaldehyde-induced paw edema in Wistar rats, in a dose-dependent manner up to 0.2 ml/kg body weight^74^. Al-Mijalli et al.^75^ demonstrated that the EO of L. nobilis have in vitro anti-inflammatory effect by inhibiting 5-Lipoxygenase (5-LOX) enzyme with IC_50_ value 48.31 ± 0.07 µg/ml, and it also significantly suppressed carrageenan-induced paw edema in Wistar rats with 70.59% inhibition at 50 mg/kg body weight. Jaradat et al.^45^ exhibited that the EO of L. nobilis showed an anti-inflammatory effect by inhibiting cyclooxygenase (COX) enzymes and exhibited 89.5 and 87.8% inhibition of COX-1 and COX-2 enzymes, respectively, at 40 µg/ml.

The EO of C. citratus exhibited a strong cytotoxic effect against RAW264.7 cells. This finding was agreement with a previous study by Sepúlveda-Arias et al.^76^ which showed the EO of C. citratus and citral exhibited a cytotoxic effect toward murine macrophage cells (RAW264.7). This toxicity prohibited further testing of the EO of C. citratus as anti-inflammatory activity should be at non-toxic concentrations. In addition, in our previous publication, we characterized the chemical composition and cytotoxic properties of the EOs under investigation, and demonstrated that the EO of C. citratus had a highly cytotoxic effect towards all evaluated cell lines; triple-negative breast cancer (MDA-MB-231), non-small lung adenocarcinoma (A549), colon adenocarcinoma (Caco2), hepatocellular carcinoma cells (HepG2) and doxorubicin-resistant hepatocellular carcinoma (HepG2/DOX)^54^.

We were unable to find any publications examining the cancer chemopreventive effects of essential oils distilled from the plant species under investigation. Other species within these genera, however, have been investigated. Also, to the best of our knowledge, there are no previous studies that have demonstrated the role of essential oil in cancer chemoprevention via the activated Nrf2 signaling pathway in murine hepatoma cells (Hepa1c1c7) model. While, it is evaluated through other models and techniques.

In a study by Li et al.^77^ Rosemary essential oil demonstrated the ability to alleviate oxidative stress by activating the Nrf2 signaling pathway, resulting in increased protein expression of NQO1 and other antioxidant enzymes such as HO-1.

The stimulation of the Nrf2-Keap1 signaling pathway is principally responsible for the induction of NQO1 by EO of Artemisia vulgaris and eucalyptol, which is a major compound. This route is essential for modulating the expression of several antioxidant enzymes, including NQO1. It also participates in the liver’s detoxification activities, so safeguarding against oxidative stress and hepatic injury^78^.

The regulation of the Nrf2 signaling pathway by essential oils offers a promising approach to augment cellular defense systems against oxidative stress. By augmenting the production of pivotal enzymes such as NQO1 and HO-1, these natural substances may provide therapeutic advantages in the management of illnesses linked to oxidative damage. Additional research is necessary to thoroughly clarify their mechanisms and their clinical uses.

Conclusion

Anti-inflammatory activity was evaluated using RAW264.7 macrophages. The EO of A. abrotanum exhibited strong anti-inflammatory potential against LPS-induced NO on RAW264.7 cells. On the other hand, a cancer chemo-preventive influence was assessed in vitro utilizing Hepa1c1c7 murine carcinoma cells and showed that the EOs of L. dentata and A. abrotanum have moderate potency to induce protein expression of NQO1 and HO-1. These findings establish of L. dentata and A. abrotanum as promising chemopreventive and anti-inflammatory species for therapeutic development.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary Material 1