# Genome mining and functional characterization of four type I sesquiterpene synthases from the Tiger Milk Mushroom Lignosus rhinocerus

## TL;DR

Scientists identified and tested four enzymes in a medicinal mushroom that produce new terpenes, expanding knowledge of its chemical properties.

## Contribution

The study functionally characterizes four new sesquiterpene synthases from Lignosus rhinocerus and identifies 11 novel sesquiterpenes.

## Key findings

- Four type I sesquiterpene synthases (LrhTS1−LrhTS4) were characterized and shown to produce 11 sesquiterpenes.
- Compounds 1−10 are newly reported from the Lignosus genus.
- LrhTS1 and LrhTS3 showed high specificity, while LrhTS2 and LrhTS4 produced multiple products.

## Abstract

The Tiger Milk mushrooms (Lignosus spp.) have long been used as traditional folk medicines throughout Southeast Asia. However, the chemical constituents of these species remain largely unexplored. In this study, four type I sesquiterpene synthases (LrhTS1−LrhTS4) from Lignosus rhinocerus TM02® were functionally characterized through genome mining, in vitro enzymatic assays, and heterologous expression in an Escherichia coli host engineered to overexpress an exogenous mevalonate pathway. The enzyme products were analyzed by GC−MS and further purified and structurally elucidated by NMR spectroscopy. A total of 11 sesquiterpenes (1−11) were identified, among which two were unstable and converted to stable derivatives (2 to 2a and 7 to 7a). Notably, compounds 1−10 are reported from the genus Lignosus for the first time. LrhTS1 and LrhTS3 exhibited high catalytic specificity, whereas LrhTS2 and LrhTS4 displayed product promiscuity. This work expands the knowledge of terpene synthase from mushroom, and advances the understanding of the chemical basis underlying the bioactivity of the Lignosus rhinocerus TM02®.

The online version contains supplementary material available at 10.1007/s13659-025-00578-9.