# Silkworm (Bombyx mori L.) pupae meal supplementation: Effect on growth performance, carcass traits, meat quality, and economic efficiency in rabbits

**Authors:** Hanan A. M. Hassanien, Ayman Hassan, Mohammed E. Gad, Noura Gouda, Hamza M. Kamel, Nabila M. El-Kassas, Mohamed A. Radwan, Usama Nayel, Abdelfattah Z. M. Salem

PMC · DOI: 10.1007/s11250-026-04863-8 · Tropical Animal Health and Production · 2026-02-02

## TL;DR

Adding silkworm pupae meal to rabbit feed improves growth and meat fat content without harming health.

## Contribution

This study shows that up to 1% silkworm pupae meal can be safely added to rabbit diets for better growth and meat quality.

## Key findings

- Rabbits fed silkworm pupae had improved body weight and feed conversion.
- Meat from SP-fed rabbits had higher fat content.
- Blood parameters and kidney function remained normal with SP supplementation.

## Abstract

This study investigated how consuming silkworm pupae (SP) meal affected rabbits that were still growing. Forty-five weaned male New Zealand White rabbits, averaging 680 g, were allocated randomly to three groups: a control group (SP0) and two groups that had SP 0.5% (SP0.5) and 1% (SP1). The SP exhibited a significant enhancement in body weight, average daily increase, and feed conversion ratio (P < 0.05). The amount of feed each group consumed stayed the same. The digestibility of dry matter, organic matter, crude fiber, and nitrogen-free extract did not change, but the digestibility of crude protein and ether extract, and the amount of nitrogen retained were all much greater (P < 0.05) in rabbits fed SP. There were no significant differences between carcass weight, slaughter body weight, and dressing %. The amount of protein, moisture, and ash in the meat was the same, but the amount of fat was greater (P < 0.05) in the SP groups. The pH of the cecum, the levels of ammonia nitrogen, acetate, butyrate, and the acetate-to-propionate ratio stayed the same, but the total volatile fatty acids went up a lot (P < 0.05) in rabbits that ate SP. The SP also caused levels of total plasma protein, albumin, and globulin to rise by a lot. The kidneys (urea and creatinine) worked just fine. It was concluded that adding up to 1% dried silkworm pupae to rabbit meals does not hurt their growth or blood parameters.

## Linked entities

- **Species:** Oryctolagus cuniculus (taxon 9986)

## Full-text entities

- **Genes:** GPx [NCBI Gene 692548], Sod1 (Superoxide dismutase 1) [NCBI Gene 692639] {aka BmSOD1, Cu/Zn-SOD, SOD, inx2}, Albumin [NCBI Gene 100009195], SP1 [NCBI Gene 100351317], SP1 (Sp1 transcription factor) [NCBI Gene 395303], SP1 [NCBI Gene 100134931]
- **Diseases:** haemorrhage (MESH:D006470), weight gain (MESH:D015430)
- **Chemicals:** butyrate (MESH:D002087), lipid (MESH:D008055), tocopherols (MESH:D024505), fatty acid (MESH:D005227), Calcium (MESH:D002118), alpha-linolenic acid (MESH:D017962), Co (MESH:D003035), Nitrogen (MESH:D009584), ether (MESH:D004986), riboflavin (MESH:D012256), orthophosphoric acid (MESH:C030242), chitosan (MESH:D048271), glucose (MESH:D005947), HCl (MESH:D006851), propionate (MESH:D011422), Mg (MESH:D008274), EE (MESH:D004997), amino acids (MESH:D000596), 2NH3-N (-), vitamin B1 (MESH:D013831), thiobarbituric acid (MESH:C029684), NA (MESH:D012964), magnesium sulfate (MESH:D008278), creatinine (MESH:D003404), VFA (MESH:D005232), n-3 fatty acids (MESH:D015525), mercuric chloride (MESH:D008627), 5N (MESH:C005072), Fe (MESH:D007501), n-6 fatty acids (MESH:D043371), lignin (MESH:D008031), biotin (MESH:D001710), Zn (MESH:D015032), Phosphorus (MESH:D010758), thiobarbituric acid reactive substances (MESH:D017392), vitamin A (MESH:D014801), petroleum ether (MESH:C004544), acetate (MESH:D000085), potassium (MESH:D011188), Unsaturated fatty acids (MESH:D005231), acid (MESH:D000143), urea (MESH:D014508), Cu (MESH:D003300), acetic (MESH:D019342), Manganese (MESH:D008345), phospholipids (MESH:D010743), I (MESH:D007455), cholesterol (MESH:D002784), campesterol (MESH:C021273), slaked lime (MESH:D002126), bile acid (MESH:D001647), vitamin E (MESH:D014810), triglycerides (MESH:D014280), vitamin D (MESH:D014807), polysaccharides (MESH:D011134), H2SO4 (MESH:C033158), pantothenic acid (MESH:D010205), Water (MESH:D014867), beta-sitosterol (MESH:C025473)
- **Species:** Camelina (genus) [taxon 71323], Bombyx mori (domestic silkworm, species) [taxon 7091], Gallus gallus (bantam, species) [taxon 9031], Sus scrofa (pig, species) [taxon 9823], Glycine max (soybean, species) [taxon 3847], Homo sapiens (human, species) [taxon 9606], Oryctolagus cuniculus (domestic rabbit, species) [taxon 9986]

## Full text

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## Figures

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## References

2 references — full list in the complete paper: https://tomesphere.com/paper/PMC12864273/full.md

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Source: https://tomesphere.com/paper/PMC12864273