# Rapid diagnostic value of next-generation sequencing-based technologies in childhood pneumonia

## TL;DR

Next-generation sequencing (NGS) offers faster and more accurate diagnosis of childhood pneumonia compared to traditional methods.

## Contribution

The study demonstrates NGS's superior diagnostic speed and accuracy in identifying pneumonia pathogens in children.

## Key findings

- NGS identified pathogens in 86.2% of cases, significantly higher than 55.3% with traditional methods.
- NGS results were available within 24 hours, much faster than 3–5 days for bacterial cultures.
- Combining NGS with albumin levels may improve pneumonia screening and clinical outcomes.

## Abstract

This study evaluates the diagnostic efficacy of next-generation sequencing (NGS) in pediatric patients with suspected pneumonia and unidentified etiologies.

This retrospective study encompassed pediatric patients with suspected pneumonia, spanning the period from January 2022 to December 2023. Nasal swabs and blood samples were collected for a comprehensive diagnostic panel, including NGS, blood culture, complete blood count, and serum biomarkers.

Routine diagnostic tests were compared with NGS for turnaround time and diagnostic accuracy. Patients were categorized based on clinical diagnosis into non-pneumonia and pneumonia groups. Logistic regression analysis was performed to identify independent predictors of pneumonia.

NGS provided results within 24 h, significantly faster than conventional bacterial cultures (3–5 days). The positivity rate for pathogen identification increased from 55.3% with traditional methods to 86.2% with NGS (p < 0.05). Serum levels of procalcitonin, creatinine, and C-reactive protein were elevated in pneumonia patients, while albumin levels were decreased. Logistic regression identified C-reactive protein and albumin as independent predictors of pneumonia. The area under the receiver operating characteristic curve for NGS was superior to conventional methods and serum biomarkers alone or in combination.

NGS is a promising tool for rapid and accurate etiologic diagnosis of pneumonia in children. The combination of NGS with albumin levels may serve as an effective screening strategy, potentially enhancing clinical management through earlier intervention and targeted therapy. Further validation in larger cohorts is warranted to establish the clinical utility of this approach.