# A Modular and Convergent “Stick and Click” Conjugation Platform Enables Fast Antibody Conjugate Library Synthesis

## TL;DR

This paper introduces a new platform for quickly making antibody drug conjugates by combining different payloads and conjugation methods in a modular way.

## Contribution

A modular and convergent conjugation platform is introduced for efficient and flexible synthesis of antibody conjugate libraries.

## Key findings

- A convergent synthesis approach was used to assemble a library of antibody conjugates with varied payloads and conjugation methods.
- The platform allows for cleavable and noncleavable linker technologies to be integrated seamlessly.
- The method enables direct comparison and targeted optimization of conjugate properties.

## Abstract

The preparation of antibody drug conjugates (ADC) most often relies on a linear sequence to elaborate the small molecule component, followed by a final bioconjugation step to attach it to its immunoglobulin partner. This linear and iterative approach is incompatible with expedient parallel synthesis and process automation. Here, we describe the design and implementation of a general modular platform for the assembly of ADCs that enables facile variation in the nature of the payload, the linker composition, and the type of bioconjugation technique used. A library of antibody conjugates bringing together several different antibodies and payloads was prepared in a convergent fashion using a range of conjugation methods, as well as cleavable or noncleavable linker technology. Aside from offering a direct comparison of different conjugation method performances, this approach enables a more targeted optimization strategy of conjugate properties by deconvoluting bioconjugation and payload attachment.