# Interspecies rice versus Arabidopsis thaliana protein–protein interactome profiling by touch‐down overlapping PCR coupled with HiFi long‐read sequencing

## TL;DR

This paper introduces a new high-throughput method to identify protein interactions between rice and Arabidopsis plants.

## Contribution

A novel method combining touch-down overlapping PCR and HiFi sequencing is developed for interspecies protein interaction profiling.

## Key findings

- The method identified 7,726 high-confidence protein interactions between rice and Arabidopsis.
- The approach integrates a library-vs-library yeast two-hybrid strategy with optimized PCR and sequencing.
- The method is effective for large-scale profiling of interspecies protein interactions.

## Abstract

Touch‐down overlapping PCR coupled with HiFi long‐read sequencing, a high‐throughput method for large‐scale profiling of protein‐protein interactions based on stitch‐PCR identified 7,726 high‐confidence interactions between rice and Arabidopsis proteins by integrating a library‐vs‐library yeast two‐hybrid strategy with optimized PCR and long‐read sequencing.