# Histopathological and immunohistochemical characterization of lesions in the golden Syrian hamster model of Nipah virus infection (Bangladesh strain)

## TL;DR

This study examines the effects of Nipah virus-Bangladesh strain in hamsters, comparing its pathology and immune response to the Malaysian strain.

## Contribution

The study provides new insights into the histopathological and immunological differences between Nipah virus-Bangladesh and Malaysian strains in a hamster model.

## Key findings

- NiV-B-infected hamsters showed similar lung pathology and viral RNA distribution to NiV-M-infected animals, with the lung as the main affected organ.
- Pulmonary lesions in NiV-B-infected animals showed high cell death, proliferation, and immune cell infiltration.
- NiV-B caused differential upregulation of IL-6 in the lungs compared to NiV-M.

## Abstract

Nipah virus (NiV) is recognised as a priority pathogen with pandemic potential by the World Health Organisation (WHO). The NiV-Bangladesh strain (NiV-B) has been associated with recent outbreaks in different districts of Bangladesh and the state of Kerala (India), and it is suggested to be more pathogenic and lethal than the NiV-Malaysian strain (NiV-M). In this study, we aimed to describe the clinical signs and pathology of NiV-B using the golden Syrian hamster model following intranasal (IN) and intraperitoneal (IP) inoculation with different doses and to compare with prior NiV-M results. For this purpose, we selected samples from NiV-B-infected animals that were submitted for H&E evaluation, immunohistochemistry (IHC), in situ hybridisation (ISH) (RNAscope technique), and multiplex immunofluorescence (mIF). The absence of neurological signs was observed in NiV-B-infected animals compared with those that were NiV-M-infected. Except for the brain, which did show only mild lesions, histopathological analysis of NiV-B demonstrated similar pathology and viral RNA in the lung, spleen, and liver compared to those of NiV-M infected animals, with the lung being the main affected organ. Pulmonary lesions consisted of areas of broncho-interstitial pneumonia associated with high cell death activation (caspase-3), proliferation (Ki67), and abundant intralesional macrophages (Iba1) and T cells (CD3). Differential upregulation of the cytokine IL-6 was observed in the lung from NiV-B compared with NiV-M infected animals. Moreover, we demonstrated the wide distribution of the NiV receptor ephrin B2 in endothelial cells, neurons, smooth muscle, epithelial cells, macrophages/type II pneumocytes, and T cells.