# Ginkgo biloba extract suppresses hepatocellular carcinoma progression by inhibiting the recruitment of myeloid-derived suppressor cells through reduced CXCL1 secretion via SRC downregulation

## TL;DR

Ginkgo biloba extract may help treat liver cancer by reducing harmful immune cell recruitment through a specific molecular pathway.

## Contribution

This study reveals a novel mechanism by which GBE suppresses HCC via SRC downregulation and reduced CXCL1 secretion.

## Key findings

- GBE treatment inhibited tumor growth and reduced MDSC recruitment in a mouse model of HCC.
- SRC was identified as a key target of GBE, with its downregulation leading to reduced CXCL1 secretion.
- SRC overexpression reversed the antitumor effects of GBE, confirming its role in the mechanism.

## Abstract

Hepatocellular carcinoma (HCC) remains one of the leading causes of cancer-related mortality worldwide, characterized by poor prognosis and limited therapeutic efficacy. Ginkgo biloba extract (GBE) has demonstrated antitumor potential, yet its precise molecular mechanisms in HCC are not fully understood.

This study aimed to elucidate how GBE suppresses HCC progression and to explore its underlying molecular mechanisms.

A subcutaneous HCC mouse model was established to evaluate the antitumor effects of GBE in vivo. Network pharmacology, molecular docking, and in vitro assays were integrated to identify and validate the core molecular targets of GBE.

GBE treatment significantly inhibited tumor growth and reduced myeloid-derived suppressor cells (MDSCs) recruitment within the tumor microenvironment. Network pharmacology identified proto-oncogene tyrosine-protein kinase (SRC) as a key target of GBE. Molecular docking revealed strong spontaneous binding affinity between active GBE components and SRC. In vitro experiments confirmed that GBE markedly downregulated SRC expression and CXCL1 secretion in HCC cells, whereas SRC overexpression reversed these effects. Clinical data further showed that SRC was upregulated in HCC tissues and correlated with poor prognosis and elevated MDSCs infiltration.

GBE suppresses HCC progression by downregulating SRC expression, which consequently reduces CXCL1 secretion and limits MDSCs recruitment within the tumor. These findings highlight GBE as a promising adjuvant immunotherapeutic strategy for HCC.