# Arsenic efflux mechanisms in ectomycorrhizal mushrooms Hebeloma bulbiferum and Hebeloma sinapizans

## TL;DR

This study explores how two mushroom species handle arsenic by identifying and testing genes that help them expel the toxic metalloid.

## Contribution

The study identifies and functionally characterizes HbACR3 and HsACR3 genes in Hebeloma mushrooms for arsenic efflux.

## Key findings

- HbACR3 and HsACR3 are plasma membrane proteins that confer arsenic resistance and reduce intracellular accumulation.
- Arsenate exposure strongly upregulates HbACR3 and HsACR3 gene expression in Hebeloma species.
- Acr3 genes are predominantly found in mushrooms and are crucial for eukaryotic arsenic detoxification.

## Abstract

Arsenic (As) is a toxic metalloid widespread in the environment, and many organisms have evolved mechanisms to mitigate its toxic effects. Bioinformatic analyses revealed that acr3 genes are predominantly distributed in mushrooms, highlighting their evolutionary and functional importance in eukaryotic arsenic metabolism. In this study, two homologous genes, HbACR3 and HsACR3, from the mushrooms Hebeloma bulbiferum and Hebeloma sinapizans were identified and functionally characterized. Both encode 399-amino-acid membrane proteins showing 99% sequence identity to each other and substantial similarity to previously characterized ACR3-type arsenite transporters from plants, yeasts, and bacteria. Heterologous expression of HbACR3 and HsACR3 in a Saccharomyces cerevisiae arr3Δ mutant restored resistance to arsenite and arsenate and significantly reduced intracellular arsenic accumulation. Fluorescence microscopy of GFP-tagged HbACR3 and HsACR3 confirmed their localization to the plasma membrane, consistent with an efflux transport function. Exposure of H. bulbiferum and H. sinapizans mycelia to arsenate led to a significant but differential transcriptional upregulation of both genes. This work provides new insight into the evolution, distribution, and physiological significance of ACR3 transporters in eukaryotic arsenic homeostasis.

Acr3 genes are widespread in fungi, indicating a key role in arsenic detoxification.HbACR3 and HsACR3 reduce cellular arsenic and confer As(III) tolerance.Arsenate exposure strongly induces HbACR3 and HsACR3 gene expression.

Acr3 genes are widespread in fungi, indicating a key role in arsenic detoxification.

HbACR3 and HsACR3 reduce cellular arsenic and confer As(III) tolerance.

Arsenate exposure strongly induces HbACR3 and HsACR3 gene expression.

The online version contains supplementary material available at 10.1007/s00253-026-13710-7.