Mapping cellular vulnerability in Parkinson’s disease using retro-AAVs and preformed α-synuclein fibrils

TL;DR

This study explores why some brain cells are vulnerable to Parkinson's disease pathology while others are not, using a mouse model and viral tracing techniques.

Contribution

The study identifies cell-autonomous factors influencing vulnerability to α-synuclein pathology in Parkinson's disease.

Findings

α-synuclein pathology spreads along anatomical pathways but does not affect all connected neurons equally.

Neurons with larger axonal arbors and higher mitochondrial oxidation levels are more vulnerable to α-synuclein accumulation.

Certain brain regions act as 'super-seeders', promoting widespread pathology propagation.

Abstract

Parkinson disease (PD) is characterized by progressive neuronal loss within defined brain regions, accompanied by α-synuclein (αSyn)-rich inclusions, termed Lewy pathology (LP). However, it is unclear which cellular factors render certain neuronal populations vulnerable, while others stay devoid of LP throughout the course of disease.

This study aimed to identify and compare the cellular architecture of vulnerable and non-vulnerable neurons exposed to αSyn pathology by using a projection-based retro-AAV approach in combination with an in vivo α-synucleinopathy mouse model. To do so, a set of viral genetic, immunohistochemical, and optical tools was used in combination with the preformed αSyn fibril (PFF) model.

αSyn pathology propagated robustly into the input connectome of the pedunculopontine nucleus (PPN). However, we observed a marked mismatch between the anatomically expected and the actual distribution of pathology. While anatomically connected neurons in the bed nucleus of the stria terminalis (BST) and the central amygdala (CEA) accumulated substantial αSyn pathology, equally strong connected neurons of the substantia nigra pars reticulata (SNr), and the dentate nucleus (DN) were devoid of pathology. Second, cellular vulnerability and resilience were consistent and reproducible features. When PFFs were injected into alternative major output projection sites of BST, CEA, SNr, and DN, we observed similar patterns of αSyn accumulation. Third, projection-specific axonal mapping revealed that the αSyn-accumulating BST and CEA neurons possessed larger axonal arbors than the more resilient neurons in SNr and DN. Correspondingly, neurons in BST and CEA exhibited higher basal mitochondrial oxidation levels, indicating an increased bioenergetic burden. Finally, the site of initial seeding significantly influenced the extent of developing brain-wide pathology, suggesting that certain brain regions may function as "super-seeders", promoting widespread propagation of pathology, while others contribute relatively little to the global LP burden.

αSyn pathology propagates along anatomical pathways, but cell-autonomous factors determine if a neuron exposed to misfolded αSyn will develop Lewy-like pathology or not.

The online version contains supplementary material available at 10.1186/s40035-026-00535-7.

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