# Genome analysis of a cluster EF bacteriophage LordBart isolated from soil in Tennessee

**Authors:** Sergei Markov, Cynthia Fecteau, Torrie Jones, Matthew Lee, Mercedes Thornton

PMC · DOI: 10.17912/micropub.biology.001929 · 2026-01-15

## TL;DR

A new soil bacteriophage called LordBart was discovered in Tennessee, with a genome of 56,975 base pairs and unique genetic features.

## Contribution

The paper presents the genome analysis of a newly isolated siphovirus bacteriophage, LordBart, grouped in cluster EF.

## Key findings

- LordBart has a genome of 56,975 bp with 86 predicted protein-coding genes.
- Eight copies of a conserved 12 bp sequence motif were found upstream of some genes of unknown function.
- The phage is classified as a siphovirus and grouped in cluster EF based on gene content similarity.

## Abstract

Bacteriophage LordBart was isolated from a soil sample in Clarksville, TN using the bacterium
Microbacterium foliorum.
The bacteriophage has a 56,975 bp genome with 86 predicted protein-coding genes, of which 32 were assigned predicted functions. LordBart has a siphovirus morphology and is grouped with bacteriophages in cluster EF based on gene content similarity. Its genome includes eight copies of a conserved 12 bp sequence motif located upstream of predicted translational start codons of some genes of unknown functions.

## Linked entities

- **Species:** Microbacterium foliorum (taxon 104336)

## Full-text entities

- **Species:** Bacteriophage sp. (species) [taxon 38018], Microbacterium foliorum (species) [taxon 104336]

## Figures

1 figure with captions in the complete paper: https://tomesphere.com/paper/PMC12859693/full.md

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Source: https://tomesphere.com/paper/PMC12859693