# ALKBH5/YTHDF2 Axis Regulates Osteogenic Differentiation Through Mediating the m6A Modification of ELK1

## TL;DR

This study explores how m6A methylation affects bone formation in osteoporosis, identifying a key pathway involving ALKBH5, YTHDF2, and ELK1.

## Contribution

The study identifies a novel regulatory axis involving ALKBH5, YTHDF2, and ELK1 in osteogenic differentiation during osteoporosis.

## Key findings

- ALKBH5 is downregulated in osteoporosis and suppresses osteogenic differentiation.
- YTHDF2 acts as an m6A reader for ALKBH5-mediated demethylation of ELK1 mRNA.
- ALKBH5/YTHDF2 axis regulates bone formation through ELK1, offering a potential therapeutic target for osteoporosis.

## Abstract

This study aimed to investigate the role of N6‐methyladenosine (m6A) methylation in osteogenic differentiation during osteoporosis (OP). Serum specimens were obtained from 25 individuals diagnosed with OP and 25 age‐matched healthy controls. In parallel, MC3T3‐E1 preosteoblastic cells were employed for in vitro functional assays. Expression levels of m6A‐associated genes were quantified using qPCR. Osteogenic potential was evaluated by measuring ALP activity with an ALP assay kit and by assessing matrix mineralization through Alizarin Red S staining. RIP and dual‐luciferase reporter assays were performed to elucidate the molecular interactions involved. To corroborate the in vitro observations, an ovariectomized (OVX) mouse model of OP was established for in vivo validation. The results revealed a significant downregulation of AlkB Homolog 5 (ALKBH5) in both serum samples from OP patients and MC3T3‐E1 cells undergoing osteogenic differentiation. Moreover, enforced expression of ALKBH5 suppressed osteogenic differentiation in these cells. Mechanistically, ELK1 was found to be a key downstream effector of ALKBH5. Additionally, YTH domain family protein 2 (YTHDF2) was demonstrated to function as the m6A reader that specifically recognizes the ALKBH5‐mediated demethylation site on ELK1 mRNA. Rescue experiments confirmed that ELK1 overexpression or YTHDF2 knockdown promoted osteogenic differentiation, whereas these effects were abolished by ALKBH5 overexpression or ELK1 silencing. In OVX mice, ALKBH5 knockdown mitigated bone loss, improved bone strength, and restored ELK1 expression. Notably, ELK1 inhibition reversed the protective effects of YTHDF2 knockdown on bone loss and mechanical strength in OVX mice. In conclusion, ALKBH5/YTHDF2 axis might be involved in osteogenic differentiation via regulating ELK1 (a key downstream effector), which might provide a new insight for OP treatment.