# Amino acid starvation and iron limitation facilitate the biofilm formation of Klebsiella pneumoniae within urine

## TL;DR

Klebsiella pneumoniae forms stronger biofilms in urine due to amino acid starvation and iron limitation, which affect key signaling pathways and gene expression.

## Contribution

Identifies amino acid starvation and iron limitation in urine as key drivers of K. pneumoniae biofilm formation through c-di-GMP signaling and EPS biosynthesis.

## Key findings

- K. pneumoniae forms more biofilms in urine than in nutrient-rich medium.
- Amino acid starvation in urine modulates c-di-GMP signaling to promote biofilm formation.
- Iron limitation in urine enhances exopolysaccharide gene expression and biofilm development.

## Abstract

Biofilm formation is a critical virulence mechanism in pathogens such as Klebsiella pneumoniae, a Gram-negative, encapsulated bacterium that has emerged as a zoonotic threat capable of infecting both humans and animals. Its biofilm-forming ability is closely associated with catheter-related and urinary tract infections. Given its potential to cross species barriers and cause significant public health concern, elucidating the environmental cues and conserved molecular pathways driving biofilm formation is essential for developing cross-species prevention strategies. Here we found that K. pneumoniae exhibited significantly greater biofilm-forming efficiency in urine than in nutrient-rich medium under comparable biomass conditions. Transposon-insertion sequencing (Tn-seq) identified 19 fitness genes essential for optimal growth in urine, most involved in the de novo biosynthesis of amino acids, particularly arginine, methionine, and isoleucine. Urine represents an amino acid-starved (AAS) environment for K. pneumoniae, modulating c-di-GMP signaling to promote biofilm formation. Eight diguanylate cyclase (DGC, c-di-GMP synthesis) genes, four phosphodiesterase (PDE, c-di-GMP degradation) genes, and four DGC + PDE genes were significantly regulated in response to urine. Furthermore, transcriptomic analysis comparing K. pneumoniae grown in urine with that grown in M9 medium revealed significant activation of genes associated with exopolysaccharide (EPS) biosynthesis, including those encoding lipopolysaccharides (LPS), capsules, peptidoglycan, and enterobacterial common antigen (ECA). Notably, K. pneumoniae increases EPS biosynthesis under the iron-limited conditions in urine, further promoting biofilm development. In conclusion, AAS-mediated c-di-GMP signaling and iron limitation are key drivers of biofilm formation by K. pneumoniae in urine, providing mechanistic insights that may guide strategies to disrupt biofilm formation.

•K. pneumoniae exhibits greater biofilm-forming efficiency in urine than medium.•Amino acid starvation in urine modulates c-di-GMP to promote biofilm formation.•Urine triggers genes for exopolysaccharide biosynthesis, enhancing biofilm matrix.•Iron limitation in urine enhances EPS gene expression and biofilm formation.

K. pneumoniae exhibits greater biofilm-forming efficiency in urine than medium.

Amino acid starvation in urine modulates c-di-GMP to promote biofilm formation.

Urine triggers genes for exopolysaccharide biosynthesis, enhancing biofilm matrix.

Iron limitation in urine enhances EPS gene expression and biofilm formation.