Augmented emicizumab-driven coagulation potential in hemophilia A state by in vitro and in vivo supplementation of combined factors IX and X

TL;DR

Adding factors IX and X can improve blood clotting in hemophilia A patients treated with emicizumab.

Contribution

This study shows combined factor IX and X supplementation enhances emicizumab's coagulation potential in hemophilia A.

Findings

Combined FIX and FX supplementation improved coagulation parameters in emicizumab-treated plasma to normal levels.

In mice, additional FIX and FX reduced clotting time and blood loss in emicizumab-treated hemophilia A models.

Thrombotic markers remained largely unchanged with combined FIX and FX supplementation.

Abstract

Persons with hemophilia A (HA) and inhibitors undergoing emicizumab prophylaxis require bypassing agents when breakthrough bleeding occurs. Recent studies have demonstrated that either factor (F)IX or FX supplementation can improve coagulation potential of emicizumab-treated persons with HA and inhibitors.

This study assessed the effect of combined supplementation with FIX and FX on the coagulation potential in emicizumab-treated HA state.

FVIII-deficient plasmas were spiked with emicizumab (50 μg/mL), FX (100 IU/dL), and various FIX levels (100-1600 IU/dL). Plasmas from emicizumab-treated persons with HA and inhibitors were also added with FIX/FX (100 IU/dL each). Coagulation potential was assessed by maximum coagulation velocity (Ad|min1|) using tissue factor (TF)/ellagic acid–triggered clot waveform analysis and peak thrombin (PeakTh) using TF-triggered thrombin generation assay. For in vivo method, emicizumab (3 mg/kg) and human (h)FIX/hFX (100 IU/kg each) were intravenously administered to HA mice (emicizumab-HA mice). Coagulation potentials in these mice with or without additional hFIX/hFX (100 IU/dL each) were assessed by clotting time plus clot formation time (CT + CFT) and blood loss using rotational thromboelastometry and tail-clip assay.

Ad|min1| and PeakTh in FVIII-deficient plasmas with emicizumab, FIX, and FX increased in FIX dose dependently. Addition of FIX and FX (100 IU/dL each) to emicizumab-supplemented FVIII-deficient plasma and plasma of emicizumab-treated persons with HA and inhibitor improved both parameters to normal levels. CT+CFT and blood loss in emicizumab-HA mice with additional hFIX/hFX (100 IU/dL each) administration were significantly shorter and decreased than those in emicizumab-HA mice. The thrombotic markers largely did not change.

Combined FIX and FX supplementation could enhance coagulation potential in emicizumab-treated persons with HA and inhibitors.

•Effect of additional factor (F)IX and FX on emicizumab-driven hemostasis is unclear.•Additional FIX and FX improved coagulation function in hemophilia A (HA) plasma spiked with emicizumab.•Administration of additional human FIX and FX enhanced emicizumab-mediated hemostasis in HA mice.•FIX and FX may further improve emicizumab-driven hemostasis in HA.

Effect of additional factor (F)IX and FX on emicizumab-driven hemostasis is unclear.

Additional FIX and FX improved coagulation function in hemophilia A (HA) plasma spiked with emicizumab.

Administration of additional human FIX and FX enhanced emicizumab-mediated hemostasis in HA mice.

FIX and FX may further improve emicizumab-driven hemostasis in HA.

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