# Toxicokinetics and tissue-specific biotransformation of modified mycotoxin zearalenone-14-glucoside (ZEN-14-G) in broilers following oral administration

## TL;DR

This study investigates how a modified mycotoxin, ZEN-14-G, is absorbed, distributed, and eliminated in broiler chickens, highlighting its conversion to a toxic form and potential health risks.

## Contribution

The study provides novel toxicokinetic data on ZEN-14-G in broilers, including its rapid conversion to ZEN and tissue distribution patterns.

## Key findings

- ZEN-14-G is rapidly hydrolyzed to ZEN in vivo with a short elimination half-life.
- ZEN was detected in multiple tissues, with highest concentrations in the liver, glandular stomach, and pectoral muscle.
- The study reveals low plasma clearance and limited distribution volume of ZEN-14-G in broilers.

## Abstract

Zearalenone-14-glucoside (ZEN-14-G), a major modified mycotoxin, has attracted considerable attention due to its potential to convert back into free zearalenone (ZEN), thereby posing toxicological risks to animals and humans. Given the limited toxicokinetic data available, this study examined the absorption, distribution, and elimination of ZEN-14-G in Arbor Acres broiler chicks following oral gavage at a dose of 0.5 mg/kg body weight to assess its potential health risks. Blood and tissue samples were collected at 0, 0.5, 1, 6, and 12 h after administration. Plasma concentrations of ZEN-14-G and its primary metabolite, ZEN, were quantified using a validated UHPLC-Q/TOF-MS method. Pharmacokinetic parameters were derived via non-compartmental analysis. The results demonstrated rapid in vivo hydrolysis of ZEN-14-G to ZEN. After oral administration, ZEN-14-G reached peak plasma concentration (Cmax) rapidly. It exhibited a short elimination half-life (T½), a limited apparent volume of distribution (Vd/F), and low total plasma clearance (CL/F). Notably, ZEN was detected in various tissues, with the highest concentrations observed in the liver, glandular stomach, and pectoral muscle. In summary, these findings underscore the important role of biotransformation in ZEN-14-G metabolism and provide key insights for assessing the health risks associated with broiler exposure to glucoside-modified mycotoxins.