# Biomass loss and composition change of energycane and biomass sorghum during aerobic and anaerobic storage

**Authors:** Yubin Yang, Tanumoy Bera, Lloyd T. Wilson, Fugen Dou, John L. Jifon, William L. Rooney, Hamid Araji, Jesse I. Morrison, Brian S. Baldwin, Joseph E. Knoll, Alan L. Wright, Dennis C. Odero, Hardev S. Sandhu, Anna L. Hale, Himaya P. Mula-Michel, Jing Wang

PMC · DOI: 10.1038/s41598-025-34190-1 · 2026-01-03

## TL;DR

This study compares how energycane and biomass sorghum lose biomass and change in composition during storage under different conditions in the southeastern US.

## Contribution

The study provides new insights into biomass storage characteristics for energycane and biomass sorghum under aerobic and anaerobic conditions.

## Key findings

- Crop type had less than 3% impact on moisture content and biomass loss, indicating similar storage behavior between energycane and biomass sorghum.
- Aerobic storage led to 49.9% dry matter loss after 9 months, while anaerobic storage resulted in 40.3% loss.
- Biomass composition changes for cellulose, hemicellulose, lignin, and ash were all less than 3% during 9 months of storage.

## Abstract

Energycane and biomass sorghum are two of the most promising cellulosic energy crops in the southeastern US. This study aims to determine the biomass loss and composition change of energycane and biomass sorghum in storage under different environments. Aerobic and anaerobic storage trials were conducted in seven locations across the southeastern US for energycane and six locations for biomass sorghum for 3, 6 and 9 months. Results revealed that crop type accounted for less than 3% of the variability in moisture content and biomass loss, suggesting similar storage characteristics between energycane and biomass sorghum. Average moisture content decreased from 60.7 to 50.4% after 9 months in covered aerobic storage piles but increased from 62.9 to 67.2% for anaerobic storage in anaerobic silage bags. Dry matter loss averaged 49.9% after 9 months of aerobic storage and 40.3% for anaerobic storage. Dry matter loss was described as a non-linear function of storage duration and average storage moisture, with greater loss for longer duration and higher storage moisture. Average changes in biomass composition for cellulose, hemicellulose, lignin, and ash were all less than 3% during the 9-month storage regardless of storage types. Results from this study provide valuable insights on changes in biomass quantity and quality during storage and fill a critical knowledge gap in addressing the challenge of year-round biomass supply to biorefineries.

## Full-text entities

- **Diseases:** Dry matter loss (MESH:D015352)
- **Chemicals:** lignin (MESH:D008031), hemicellulose (MESH:C007916)
- **Species:** Sorghum bicolor (broomcorn, species) [taxon 4558]

## Figures

8 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12855183/full.md

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Source: https://tomesphere.com/paper/PMC12855183