# Transcriptomic dataset of RAW264.7 murine macrophages pretreated with 9-methoxycanthin-6-one under poly(I:C)-TLR3 stimulation

## TL;DR

This study provides RNA sequencing data from mouse macrophages treated with a natural compound before viral-mimicking stimulation, helping to understand immune responses.

## Contribution

The study introduces a novel transcriptomic dataset of RAW264.7 macrophages treated with 9-methoxycanthin-6-one under TLR3 stimulation.

## Key findings

- The dataset includes normalized RNA-seq expression matrices and differential gene expression profiles.
- Pathway enrichment analysis reveals genes and pathways responsive to the compound under immune activation.
- The dataset supports comparative studies of antiviral transcriptional responses and macrophage activation markers.

## Abstract

This dataset presents RNA sequencing (RNA-seq) data from RAW264.7 murine macrophages pretreated with 9-methoxycanthin-6-one, a canthin-6-one–type alkaloid isolated from Eurycoma longifolia Jack, and subsequently stimulated with polyinosinic:polycytidylic acid [poly(I:C)], a synthetic double-stranded RNA analog that activates TLR3-mediated antiviral signaling. RAW264.7 cells were pretreated with 9-methoxycanthin-6-one (30 µM) for 30 min and then exposed to poly(I:C) (20 µg/mL) for 6 h. Total RNA was extracted, quality-checked, and sequenced on the Illumina platform to generate paired-end reads. Differential expression analysis and functional annotation were performed to profile genes responsive to 9-methoxycanthin-6-one treatment under poly(I:C) stimulation. The dataset includes normalized expression matrices, lists of upregulated and downregulated genes, and pathway enrichment outputs in standard formats. These data provide a reference resource for understanding the transcriptomic responses of macrophages to natural alkaloid treatment during viral-mimetic immune activation. The dataset can be reused to compare host antiviral transcriptional responses across TLR3-related pathways, evaluate macrophage activation markers, or integrate with other E. longifolia bioactive compounds.