Radiolabeled 5‑Fluorouracil-Loaded Solid Lipid Nanoparticles: A Potential Platform for Colorectal Cancer Imaging
Meliha Eki̇nci̇, İrem Mansuroğlu, Yiğit Uyanikgi̇l, Emel Öykü Çeti̇n Uyanikgi̇l, Derya İlem-Özdemi̇r
TL;DR
Researchers developed radiolabeled 5-fluorouracin-loaded nanoparticles that could be used for imaging colorectal cancer, showing good stability and strong cell association.
Contribution
The novel contribution is the development of radiolabeled 5-FU-loaded solid lipid nanoparticles for targeted colorectal cancer imaging.
Findings
The nanoparticles had a mean size below 150 nm and high radiolabeling efficiency (>90%).
The radiolabeled nanoparticles showed strong and sustained cell association with HT-29 colorectal cancer cells.
The formulations were biocompatible and exhibited hydrophilic characteristics suitable for systemic circulation.
Abstract
Radiolabeled 5-fluorouracil (5-FU)-loaded solid lipid nanoparticles (SLNs) were successfully developed using high shear homogenization and ultrasonication techniques. The SLNs were characterized for particle size, polydispersity index, and zeta potential. The formulations exhibited a mean particle size below 150 nm with narrow size distribution and negative surface charge, indicating good colloidal stability. The radiolabeling of SLNs with technetium-99m ([99mTc]Tc) was performed using stannous chloride as the reducing agent, achieving a high radiolabeling efficiency (>90%). The in vitro radiochemical stability of [99mTc]Tc-5-FU-SLNs was confirmed in saline, serum, and cell culture medium, where the radiochemical purity remained above 90% for up to 6 h. Partition coefficient studies demonstrated that the radiolabeled formulations exhibited hydrophilic characteristics, supporting their…
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Taxonomy
TopicsNanoparticle-Based Drug Delivery · RNA Interference and Gene Delivery · Advancements in Transdermal Drug Delivery