# Improved Dye-Filling Protocol for Multiple Nematode Species

## TL;DR

This paper introduces a new dye-filling method that improves neuron visualization in multiple nematode species.

## Contribution

A modified dye-filling protocol that works robustly across multiple nematode species and neuron types.

## Key findings

- The protocol improves dye-filling in amphid, phasmid, and inner labial neurons in C. elegans and P. pacificus.
- The method enables consistent visualization of male-specific ray sensory neurons and support cells.
- The technique is robust and applicable across multiple nematode species.

## Abstract

Dye-filling has been used extensively to study the development and morphology of sensory neurons in the nematode

C. elegans

. Here, we report a modified dye-filling protocol that improves the robustness of amphid, phasmid, and inner labial neuron dye-filling in

C. elegans

, as well as in the nematode satellite model
P. pacificus.
Our method also allows for the consistent visualization of male specific ray sensory neurons and associated support cells in these species.