A novel methodology to characterize beat-to-beat alternations in whole-cell calcium currents
Xavier Marimon, Miguel Cerrolaza, Carmen Tarifa, Leif Hove-Madsen
TL;DR
This paper introduces a new method to measure beat-to-beat alternations in calcium currents in heart cells using electrophysiological data.
Contribution
The paper proposes a novel 'alternation index' to quantify beat-to-beat alternations in calcium currents.
Findings
The alternation index is a robust measure for detecting alternating patterns in calcium signals (p ≤ 0.01 **).
The method showed good agreement with other features like L-type calcium current inactivation and tail current measurements.
A database of 24 patch clamp signals was used to validate the algorithm's accuracy.
Abstract
An automated high-throughput procedure to quantify the degree of electrical beat-to-beat alternants in human atrial myocytes from electrophysiological recordings of the transmembrane ion currents of calcium ions (Ca2+) is presented and discussed. The patch clamp technique in whole-cell mode was used to record the myocyte calcium signal. A database consisting of 24 patch clamp signals (N = 24) of which 13 had a uniform behaviour and 11 had an alternating behaviour, was created. Several features were computed to characterize the transmembrane ion currents: peak amplitude, time constants, and area under the ion current trace. The presented algorithm includes a feature detector whose accuracy has been validated using simulated calcium signals generated by an electrical model that accurately represents the cardiomyocyte behaviour in a patch clamp experiment. Among these calculated features,…
Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.
Click any figure to enlarge with its caption.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11
Figure 12
Figure 13
Figure 14
Figure 15
Figure 16Peer Reviews
No public reviews on file for this paper yet. If you reviewed it on a platform where reviews are public (OpenReview, ICLR, NeurIPS, ICML), you can paste yours below so the community can read it here.
Videos
No videos yet. Explain this paper in a talk, walkthrough, or lecture? Add one.
Taxonomy
TopicsCardiac electrophysiology and arrhythmias · Atrial Fibrillation Management and Outcomes · ECG Monitoring and Analysis