Chromatography's evolution, unlocking affinity's new solution: potential-controlled affinity membrane chromatography

TL;DR

A new method for purifying antibodies uses electrical potential to improve efficiency and quality, offering a sustainable alternative to traditional techniques.

Contribution

Introduces potential-controlled affinity membrane chromatography for efficient and mild antibody purification.

Findings

Up to 95% of bound antibodies can be eluted using optimal voltages of +2.5 to 3 V.

Antibody quality is preserved under mild conditions, confirmed by DLS, SEC–MALS, and SPR analyses.

Abstract

Antibody purification is key to advancing immunotherapy, but high costs in traditional affinity chromatography remain a barrier. In response, we present a breakthrough technology for purifying antibodies from human blood plasma (HBP) and cell culture supernatant using a potential-controlled method. This innovative platform applies an electrical potential to efficiently elute up to 95% of bound antibodies from Protein A affinity membranes at optimal voltages of +2.5 to 3 V, all without the need for buffer exchange. Antibody quality, analyzed via Dynamic Light Scattering (DLS), Size-Exclusion Chromatography (SEC) with Multi-Angle Light Scattering (SEC–MALS), and Surface-Plasmon-Resonance (SPR), demonstrated superior retention of antibody integrity. Our potential-controlled affinity membrane chromatography (PCAMC) offers a revolutionary alternative to traditional techniques, boosting efficiency and sustainability while overcoming the limitations of conventional affinity methods. This study sets the stage for a cost-effective and eco-friendly future in antibody therapeutics.

Potential-controlled affinity membrane chromatography enables antibody purification without changes in pH or salt concentration, supporting mild processing conditions and improved antibody quality.

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