# Spatial and temporal dynamics of cancer-associated fibroblast niches in breast cancer

## TL;DR

This study explores how cancer-associated fibroblasts form different niches in breast cancer and how their spatial arrangement affects tumor progression.

## Contribution

The study identifies six CAF substates and their spatial organization in breast cancer, revealing functional niches that influence tumor-immune interactions.

## Key findings

- Six distinct CAF substates were identified using single-cell RNA-sequencing and immunostaining.
- Immunomodulatory CAFs co-localize with immune cells, while myofibroblastic CAFs form a tumor shield.
- Spatial analysis showed temporal changes in stromal composition and niche-specific CAF functions.

## Abstract

Cancer-associated fibroblasts (CAFs) are the main constituents of the tumor microenvironment. Several studies have delineated CAF heterogeneity in different types of tumors, however, it is still unknown how the distinct CAF transcriptional profiles are established during tumor progression.

We reanalyzed a previously published single-cell RNA-sequencing dataset of MMTV-PyMT tumors at higher resolution using Seurat , and CytoTRACE to characterize CAF subtypes and their differentiation states. Wilcoxon rank sum test was applied for differential gene expression. Multiplex immunostaining (Akoya PhenoImager HT) was performed on 38 murine mammary tumors from MMTV-PyMT mice to identify the distinct CAF subtypes. Whole-slide imaging and spatial analysis were conducted using QuPath and Cellpose , followed by neighborhood clustering and interaction mapping with CytoMAP . Cellular distances from CAFs to immune, tumor, and endothelial cells were quantified using SPIAT and Wilcoxon tests for comparisons. In parallel, human spatial transcriptomics data from the 10X Genomics Xenium platform were integrated for cross-species validation.

Here, by single cell RNA-sequencing and multiplex immunostaining, we identify six CAF substates. Spatial analysis on immunostained murine mammary tumors and human spatial transcriptomics data outlined temporal changes in stromal composition and the existence of distinct functional niches enriched with different CAF substates. Immunomodulatory CAFs co-localized with immune cells while myofibroblastic CAFs formed a shield around the tumor core, thus preventing immune infiltration.

Our work supports the idea that distinct spatial locations dictate different CAF transcriptional programs. Targeting specific functional niches will ultimately hinder tumor progression by inhibiting signaling between distinct CAF substates and the surrounding tumor microenvironment.

The online version contains supplementary material available at 10.1186/s13058-025-02183-7.