# Therapeutic Effects of Noninvasive Electrical Stimulation in Combination Transplantation of Human Adipose‐Derived Stem Cells‐Derived Dopaminergic Neuron on the Monkey Model of Parkinson's Disease

## TL;DR

Noninvasive electrical stimulation, alone or with stem cell-derived neurons, improves Parkinson's disease symptoms in monkeys.

## Contribution

Demonstrates the therapeutic potential of noninvasive electrical stimulation combined with stem cell therapy for Parkinson's disease.

## Key findings

- NES or NES-DN increased dopamine levels and improved mitochondrial dysfunction in PD monkeys.
- The treatment reduced neuroinflammation and enhanced synaptic function while protecting TH neurons.
- NES/NES-DN may modulate the immune system by regulating serpin family A member 3.

## Abstract

Parkinson's disease (PD) is a neurodegenerative disease caused by the loss of dopaminergic neurons (DNs). Currently, there is no treatment that can cure PD. Deep brain stimulation has been used to treat PD due to its good effectiveness, but there are safety issues. Therefore, noninvasive electrical stimulation (NES) may be an effective and safe strategy for the treatment of PD. Here, we performed NES treatment and NES combined with human adipose‐derived stem cells‐induced DN transplantation (NES‐DN) on the PD monkey model to explore the therapeutic effect of NES on PD. The results show that NES or NES‐DN can increase dopamine levels, improve mitochondrial dysfunction, reduce neuroinflammation, enhance synaptic function, and protect TH neurons, thereby improving the movement disorders of PD. Moreover, NES/NES‐DN may exert immunomodulatory effects by regulating serpin family A member 3 in PD monkeys. Our results support the scientific basis and preclinical evidence for NES in the treatment of PD. Not only does NES alone improve PD, but NES combined with stem cell therapy can greatly enhance the therapeutic effect of PD.

Our results showed that noninvasive electrical stimulation (NES) alone or in combination with human adipose‐derived stem cells (hADSCs)‐induced dopaminergic neuron (NES‐DN) improved the behavioral deficits and pathological features of the MPTP‐induced PD monkey model. These findings provide a solid scientific basis and preclinical evidence for NES treatment of PD. It also provides new guidance for the combination of NES with other therapies, especially stem cell transplantation therapy.