# Quantification of eef1a1l1 transcript in early zebrafish development

## TL;DR

The study measures eef1a1l1 gene expression during early zebrafish development and finds it remains stable despite rapid transcript accumulation.

## Contribution

The paper provides absolute transcript quantification of eef1a1l1, confirming its stability as a reference gene during zebrafish development.

## Key findings

- eef1a1l1 transcripts accumulate rapidly during early zebrafish development.
- Normalized to cell number, eef1a1l1 expression remains remarkably steady.
- This stability makes eef1a1l1 suitable as an invariant internal control.

## Abstract

Eukaryotic elongation factor 1-alpha
is among the most highly expressed genes in vertebrates. Because its transcript levels are less sensitive to perturbations than those of other housekeeping genes, the zebrafish ortholog

eef1a1l1

is widely used as a reference. Here, we report absolute measurements of

eef1a1l1

transcript abundance spanning the first 36 hours of zebrafish development. We find that

eef1a1l1

transcripts accumulate rapidly; however, when normalized to cell number, its expression is remarkably steady. This distinguishes

eef1a1l1

from developmentally regulated genes and underscores its suitability as an invariant internal control.