# Zinc Sulfate Alleviates Olanzapine Induced Alteration in Hepatic Protein Patterns and Antioxidant Defense System in Rats

## TL;DR

This study shows that zinc sulfate can reduce liver damage caused by the antipsychotic drug olanzapine in rats.

## Contribution

The study demonstrates that zinc sulfate supplementation can reverse olanzapine-induced hepatic alterations in protein patterns and antioxidant defenses.

## Key findings

- Zinc sulfate reduced oxidative stress and inflammatory markers in liver tissues affected by olanzapine.
- Zinc sulfate improved electrophoretic protein and isoenzyme patterns altered by olanzapine.
- Zinc sulfate enhanced heme interactions and oxygen utilization in hemoglobin.

## Abstract

One of the atypical antipsychotic drugs is olanzapine (OLZ). Enhancing metabolic and detoxifying activities requires zinc (Zn). Thus, the objective of the current study was to determine if Zn supplementation might effectively reduce hepatic lesions induced by OLZ. The oxidative stress, inflammatory, and fibrotic indicators of the liver tissues were evaluated, and electrophoretic methods were used to analyze the native protein and isoenzyme patterns. Furthermore, the liver tissues were examined histopathologically. The dynamic motion of the extracted hemoglobin was also examined. In addition to the serious damage identified histopathologically, our results show that OLZ treatment altered the liver tissue’s antioxidant and inflammatory indicators. The zinc sulfate (ZnSO4) salt solution, given in a dose-dependent fashion, reduced these changes. Additionally, the ZnSO4 salt solution enhanced the natural protein, lipid, and calcium moieties of the protein pattern that were electrophoretically detected and changed by OLZ. In terms of the isoenzyme patterns, the ZnSO4 salt solution reduced the electrophoretic catalase (CAT), peroxidase (POX), and β-esterase (β-EST) isoenzyme patterns that were hampered by OLZ in a dose-dependent manner. Rats treated with OLZ in addition to ZnSO4 showed an increase in heme-heme interactions, suggesting that zinc therapy stabilized oxyhemoglobin. This promotes a more effective folding process that improves the use of oxygen. A dosage of 100 mg/kg of ZnSO4 was shown to normalize physiological, histological, and biochemical markers. It also improved interactions between heme molecules.

The online version contains supplementary material available at 10.1007/s12011-025-04673-3.