# Mitochondrial DNA phylogeography of a species-specific sucking louse, Johnsonpthirus heliosciuri, act as a proxy to provide insights into the population connectivity of its host, smith’s bush squirrels, Paraxerus cepapi

## TL;DR

This study uses lice DNA to understand squirrel population movement, finding that habitat fragmentation may be limiting squirrel mobility.

## Contribution

The study demonstrates that lice can act as a magnifying glass for host phylogeography due to their faster evolutionary rates.

## Key findings

- Squirrel mitochondrial DNA showed no geographic genetic structure, while lice mitochondrial DNA revealed strong geographic structure.
- Nuclear EF1 data showed no geographic structure in either squirrels or lice.
- Louse mitochondrial DNA suggests recent habitat fragmentation is limiting squirrel movement.

## Abstract

Due to co-evolution, permanent species-specific lice and their hosts often exhibit congruent phylogenetic patterns, and to a lesser extent also congruent phylogeographic structures. However, because ectoparasites generally have smaller effective population sizes (due to their aggregated distribution), and they have a faster evolutionary rate (generation time effect), their phylogeographic structures are often more pronounced. This study investigates the phylogeographic structure of a squirrel Paraxerus cepapi and one of its louse species, Johnsonpthirus heliosciuri, sampled from eight localities in South Africa. Statistical haplotype networks derived from 51 host mitochondrial DNA control region sequences revealed a lack of geographic genetic structure among sampling sites, with most genetic variation found within populations (ΦST = 0.304, P < 0.05). In stark contrast, analyses of 43 louse mitochondrial COI sequences showed a clear pattern of geographic genetic structure, with most variation occurring between populations (ΦST = 0.797, P < 0.05). Nuclear Eukaryotic Elongation Factor 1 (EF1) data revealed no geographic structure in either species. The lack of phylogeographic congruence between host and louse, as well as between mitochondrial and nuclear markers, is likely due to stochastic differences in the evolutionary rates of host and parasite DNA. In this study, the species-specific permanent parasite acted as a biological proxy, or a ‘magnifying glass’, for host phylogeography. The louse mitochondrial DNA data suggest that recent anthropogenic habitat fragmentation may indeed be limiting squirrel movement across the landscape.