# BoGHV-4 Genotypic Diversity Shapes Inflammatory and Viral Gene Expression in Platelet-Rich Plasma-Supplemented Bovine Endometrial Cells

## TL;DR

This study shows that different types of a bovine virus affect inflammation in cow uterine cells, and a treatment called PRP has limited impact on these effects.

## Contribution

The study reveals that BoGHV-4 genotypes, not PRP, primarily determine inflammatory and viral gene expression patterns in bovine endometrial cells.

## Key findings

- Genotype 07-435 caused sustained viral and cytokine gene activation, while genotype 10-154 caused early but transient inflammation.
- PRP set a common baseline for inflammation but did not override the effects of viral genotype on gene expression.
- BoGHV-4 genotypic diversity was the main factor influencing the intensity and duration of the immune response.

## Abstract

Bovine gammaherpesvirus 4 (BoGHV-4) is an opportunistic uterine pathogen whose reactivation is associated with postpartum inflammation and bacterial lipopolysaccharide (LPS). Platelet-rich plasma (PRP) is a regenerative biotherapeutic capable of modulating inflammatory responses, although its effects may vary depending on BoGHV4 genotype. In this study, primary bovine endometrial cells (BECs) were cultured in medium containing 10% PRP instead of fetal bovine serum, infected with two genetically divergent BoGHV-4 isolates (07-435, genotype 3; 10-154, genotype 2), and subsequently stimulated with bacterial lipopolysaccharide (LPS, 100 ng/mL). Expression of the viral immediate-early gene IE-2 and host immune genes (TLR4, TNF-α, CXCL8, and IFN-γ) were quantified by RT-qPCR from 4 to 48 h after stimulation. Isolate 07-435 induced a sustained activation of IE-2 and gradual cytokine upregulation, while isolate 10-154 elicited an early but transient inflammatory response followed by gene downregulation. PRP did not modify the strain-specific patterns of viral and inflammatory gene expression but established a common inflammatory baseline, whereas the magnitude and temporal profile of the response continued to be dictated by the viral genotype. These findings indicate that BoGHV-4 genotypic diversity remained the main determinant of response intensity and duration, supporting PRP as a context-dependent rather than a universal antiviral modulator.