# Dietary Supplementation with Chrysanthemum morifolium Ramat cv. ‘Hangju’ Flower Extract Alleviates Skin Photoaging in SKH-1 Hairless Mice

## TL;DR

A flower extract from Chrysanthemum morifolium helps reduce skin aging in mice by improving skin health and gut microbiota.

## Contribution

This study demonstrates the anti-photoaging effects of Chrysanthemum morifolium extract in a mouse model.

## Key findings

- CME supplementation increased skin moisture and reduced wrinkles in photoaged mice.
- CME suppressed epidermal hyperplasia and DNA damage while enhancing collagen density.
- CME altered gene expression and improved gut microbiota disrupted by UVB exposure.

## Abstract

Background/Objectives: Skin photoaging represents a predominant form of extrinsic aging, characterized by structural and functional impairment of the skin barrier. In severe cases, it may precipitate dermatological diseases and even tumors. Given the prevalence and detrimental effects of skin photoaging, strategies for its effective prevention and mitigation have garnered significant research interest. Chrysanthemum morifolium Ramat cv. ‘Hangju’ contains diverse bioactive compounds, including flavonoids, phenylpropanoids, phenolic acids, and polysaccharides, which have been proven to exhibit antioxidant and anti-inflammatory effects. Methods: This study employed a UVB-induced mouse model of skin photoaging to evaluate the potential of dietary supplementation with Chrysanthemum morifolium Ramat cv. ‘Hangju’ flower extract (CME) in vivo. Results: In the photoaged skin of female SKH-1 hairless mice, dietary supplementation with CME significantly increased skin moisture content, reduced wrinkle formation, suppressed epidermal hyperplasia, enhanced collagen density, and suppressed the senescence marker expression and DNA damage marker expression. Analysis of the skin transcriptome suggested that CME could alter gene expression patterns and potentially modulate critical signaling pathways involved in skin homeostasis. Moreover, 16S rRNA sequencing indicated that CME mitigated UVB-induced gut microbiota dysbiosis. Conclusions: These preclinical findings reveal the anti-photoaging property of dietary CME supplementation and point to its potential application as a functional dietary supplement for promoting skin health.