Manganese-oxidizing Exiguobacterium acetylicum 4-3-1 reduces cadmium accumulation in spinach

TL;DR

A manganese-oxidizing bacterium helps spinach grow better and absorb less cadmium, offering a way to reduce toxic metal contamination in crops.

Contribution

The study identifies a novel rhizobacterium that reduces cadmium uptake in spinach through both intrinsic and extrinsic mechanisms.

Findings

E. acetylicum 4-3-1 reduced Cd concentration in spinach leaves by 53.07%.

The bacterium increased spinach biomass by 184.3% and chlorophyll content by 33.99%.

It altered the rhizosphere microbiome, promoting beneficial bacteria like Bacillales.

Abstract

Cadmium (Cd) accumulation in edible plants is a significant global concern. This research explores the potential of a manganese-oxidizing rhizobacterium, Exiguobacterium acetylicum 4-3-1, to promote spinach growth while reducing Cd uptake. The bacterium produces indole-3-acetic acid and siderophores and effectively removed 73.74% of free CdCl2. Under Cd stress (10.5 mg/kg), E. acetylicum 4-3-1 significantly increased spinach biomass by 184.3% (dry weight) and chlorophyll content by 33.99%, while decreasing the Cd concentration in spinach leaves by 53.07% through both intrinsic and extrinsic mechanisms. Intrinsically, E. acetylicum 4-3-1 inoculation up-regulated pathways related to photosynthesis and energy metabolism in spinach, while down-regulating genes linked to heavy metal transport. Extrinsically, it oxidizes Mn(II) to form manganese oxides that may immobilize Cd. Moreover, inoculation with strain 4-3-1 altered the rhizosphere microbiome of spinach, increasing the presence of beneficial bacteria like Bacillales. A synthetic community (SynCom) composed of Bacillus subtilis and E. acetylicum 4-3-1 demonstrated synergistic effects on spinach growth under Cd stress. Thus, E. acetylicum 4-3-1 has the potential for Cd bioremediation in crops and promotes sustainable agriculture.

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