# Uncovering the Genetic Basis of Grain Protein Content and Wet Gluten Content in Common Wheat (Triticum aestivum L.)

## TL;DR

This study identifies genetic loci and develops markers to improve wheat quality by increasing protein and gluten content.

## Contribution

The research discovers novel genetic loci and validates KASP markers for wheat quality traits.

## Key findings

- Four loci for grain protein content were identified on chromosomes 1A, 3A, 3B, and 4B.
- Three loci for wet gluten content were found on chromosomes 4B, 5A, and 5D.
- Seven candidate genes were linked to biological pathways affecting wheat quality.

## Abstract

Improving wheat processing quality is a crucial objective in modern wheat breeding. Among various quality parameters, grain protein content (GPC) and wet gluten content (WGC) significantly influence the end-use quality of flour. These traits are controlled by multiple minor effect genes and highly influenced by environmental factors. Identifying stable and major-effect genetic loci and developing breeder-friendly molecular markers are of great significance for breeding high-quality wheat varieties. In this study, we evaluated the GPC and WGC of 310 diverse wheat varieties, mainly from China and Europe, across four environments. Genotyping was performed using the wheat 100K SNP chip, and genome-wide association analysis (GWAS) was employed to identify stable loci with substantial effects. In total, four loci for GPC were identified on chromosomes 1A, 3A, 3B, and 4B, with explained phenotypic variation (PVE) ranging from 6.0 to 8.4%. In addition, three loci for WGC were identified on chromosomes 4B, 5A, and 5D, which explained 7.0–10.0% of the PVE. Among these, three loci coincided with known genes or quantitative trait loci (QTL), whereas QGPC.zaas-3AL, QGPC.zaas-4BL, QWGC.zaas-4BL, and QWGC.zaas-5A were potentially novel. Seven candidate genes were involved in various biological pathways, including growth, development, and signal transduction. Furthermore, five kompetitive allele specific PCR (KASP) markers were developed and validated in a natural population. The newly identified loci and validated KASP markers can be utilized for quality improvement. This research provides valuable germplasm, novel loci, and validated markers for high-quality wheat breeding.