The Effects of Chinese Dwarf Cherry (Cerasus humilis) Kernel Oil on Defecation and the Gut Microbiota in Constipated Mice

TL;DR

This study shows that Chinese dwarf cherry kernel oil helps relieve constipation in mice by improving gut function and microbiota.

Contribution

The study reveals the novel constipation-relieving effects and microbiota-modulating properties of CDC kernel oil.

Findings

CDC kernel oil reduced first black fecal defecation time and increased fecal output in constipated mice.

It altered neurotransmitter levels and increased beneficial gut bacteria and short-chain fatty acids.

Histological improvements in intestinal mucus cells were observed after CDC kernel oil treatment.

Abstract

Background: The Chinese dwarf cherry (CDC) has been valued for over 2000 years for its medicinal and nutritional properties, particularly its kernels. Despite its recognition as a rich source of oil, the potential health benefits of CDC kernel oil remain unclear. Method: Initially, we evaluated the preventive effectiveness of CDC in a mouse model of constipation induced by loperamide. Results: The findings indicated that CDC kernel oil alleviated constipation by reducing the first black fecal defecation time and increasing the fecal number, wet weight, water content and gastrointestinal transit rate in model mice. Additionally, CDC kernel oil reduced inhibitory neurotransmitters and increased excitability neurotransmitters, two anti-oxidases’ activity and fecal short-chain fatty acid (SCFA) content. Histological analysis revealed an improved mucus cell morphology in the intestinal tract. Furthermore, CDC kernel oil increased the abundance of some beneficial bacteria. It was identified that the gut microbiota was associated with neurotransmitters, mediators of inflammation and SCFAs. Conclusion: The findings offer a scientific foundation for considering CDC kernel oil as a potential functional food for the alleviation of constipation.

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