# Effect of Pyroligneous Acid on Needle Retention and Certain Stress-Related Phytochemicals in Balsam Fir (Abies balsamea)

## TL;DR

This study tested pyroligneous acid to improve postharvest needle retention in balsam fir, finding no improvement in retention but some benefits to plant stress markers.

## Contribution

The novel contribution is evaluating pyroligneous acid's effect on balsam fir needle retention and stress-related phytochemicals.

## Key findings

- Pyroligneous acid did not improve postharvest needle retention in balsam fir.
- 4% pyroligneous acid increased proline concentration by 40% and reduced membrane injury by 26%.
- Needle abscission reached 100% and water uptake decreased by over 80% over 8 weeks.

## Abstract

Balsam fir is an important specialty horticultural crop in eastern North America and commonly harvested for use as Christmas trees. Postharvest quality is a major challenge for producers, who are particularly concerned about postharvest needle retention. It was hypothesized that pyroligneous acid (PA) would help increase postharvest needle retention in balsam fir when supplied via xylem or foliage. This project first identified foliar spraying as the best application method, then designed a multivariate experiment with two factors. The first factor was foliar treatment (control, water, 1% PA, 2% PA, and 4% PA). The second factor was time, where branches were evaluated for needle abscission at 0, 2, 4, 6, and 8 weeks after harvest. The experiment was replicated 5 times and needle abscission, water uptake, chlorophyll, carotenoids, flavonoids, total phenolics, membrane injury, proline, and H2O2 production were all measured in response. Postharvest abscission reached 100% over the 8-week experiment and water uptake decreased by over 80%. Chlorophyll, proline, membrane injury, and H2O2 production all increased over time. Although PA did not improve needle retention compared to the control under the tested conditions, 4% PA spray increased proline concentration by 40% while decreasing membrane injury by 26%. Ultimately, PA did not consistently improve needle retention but did induce proline accumulation and membrane protection.