# Saccharomyces cerevisiae Response to Magnetic Stress: Role of a Protein Corona in Stable Biosynthesis of Silver Nanoparticles

## TL;DR

Exposing yeast to magnetic fields helps create smaller, more stable silver nanoparticles by triggering protein secretion.

## Contribution

A novel method for stabilizing silver nanoparticles using magnetic stress-induced protein coronas in yeast.

## Key findings

- SMF exposure reduces AgNP size and enhances crystalline structure.
- SMF-exposed AgNPs show improved stability over 75 days.
- Protein coronas on AgNPs are linked to magnetic stress in yeast.

## Abstract

Saccharomyces cerevisiae was cultured under the influence of static magnetic fields (SMFs) to assess their impact on the biosynthesis of silver nanoparticles (AgNPs). Cell-free media derived from SMF-exposed cultures facilitated the formation of AgNPs, with a significant reduction in nanoparticle size observed at an optimal field strength of 7 mT. AgNPs synthesized under SMF conditions exhibited smaller crystalline structures than those produced in control media, as evidenced by dynamic light scattering (DLS) and transmission electron microscopy (TEM) measurements. Over a 75-day period, SMF-exposed AgNPs demonstrated enhanced stability, as determined by DLS and polydispersity index (PDI) assessments. Further analysis through sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Fourier transform infrared spectroscopy (FTIR) suggested the formation of a protein corona on the AgNPs in SMF-treated samples, which likely inhibits agglomeration and enhances long-term stability. These findings indicate that SMF-induced stress in S. cerevisiae triggers the secretion of specific proteins that contribute to the stabilization of AgNPs, providing a novel approach to controlling nanoparticle synthesis and stability through magnetic field exposure.