# Self-Templated Highly Porous Gold Electrodes for Antibiofouling Electrochemical (Bio)Sensors

## TL;DR

Researchers developed a porous gold electrode that resists biofouling, a common issue in biosensors, by using a self-templated method with hydrogen bubbling.

## Contribution

The novel self-templated highly porous gold film significantly enhances antibiofouling properties in electrochemical biosensors.

## Key findings

- The h-PG film has an electrochemically active surface area 88 times higher than unmodified electrodes.
- The h-PG-modified electrode showed rapid regeneration after biofouling exposure due to pore size and electrostatic effects.
- The h-PG film performed well in real serum and plasma samples, indicating biomedical potential.

## Abstract

Biofouling arises from non-specific adsorption of several components present in complex biofluids, such as full blood, on the surface of electrochemical biosensors, with a resulting loss of functionality. Most biomarkers of clinical relevance are present in biological fluids at extremely low concentrations, making antibiofouling strategies necessary in electrochemical biosensing. Here, we demonstrate the effect of a highly porous gold (h-PG) film electrodeposited on a gold screen-printed electrode (AuSPE) using a self-templated method via hydrogen bubbling as an antibiofouling strategy in electrochemical biosensor development following exposure of the electrode to bovine serum albumin (BSA) at two different concentrations (2 and 32 mg/mL). The h-PG film has a high electrochemically active surface area, 88 times higher than the AuSPE electrode, with a pore size ranging from 2 to 50 μm. A rapid decrease in the Faradaic current was observed with the unmodified AuSPE, attesting to the strong biofouling effect of BSA at both concentrations tested. Notably, the h-PG-modified electrode showed an initial peak current decline, more evident at a higher BSA concentration, followed by rapid electrode regeneration when the electrode was left idle in the biofouling solution. Similar results were obtained for unmodified and modified electrodes in real serum and plasma samples. The regeneration process, explained in terms of balance between h-PG pore size and protein size, the nanoscale architecture of the h-PG electrodes, and repulsive electrostatic forces, indicates the huge potential of the h-PG film for use in biomedical electrochemical sensing.