# Screening Heterofermentative Lactic Acid Bacteria as Silage Inoculants for Osmotolerance

## TL;DR

This study explores how to select lactic acid bacteria that can tolerate high dry matter in silage, improving silage stability.

## Contribution

A new in vitro method is proposed to assess osmotolerance in heterofermentative lactic acid bacteria for silage inoculants.

## Key findings

- Heterofermentative LAB are less affected by high dry matter and osmolality than homofermentative LAB.
- The in vitro test can qualitatively assess osmotolerance but not rank LABhe strains quantitatively.

## Abstract

Heterofermentative lactic acid bacteria (LABhe) can help to increase aerobic stability of silages. As high dry matter silages are especially prone to aerobic deterioration, the question was whether an in vitro test could help to select for LABhe with tolerance to these conditions, that is, to high osmolality. A medium proven for homofermentative lactic acid bacteria (LABho) with high sugar concentration was applied in vitro while validating the results in high DM grass silages in situ. The pH was measured at 0, 24, 36 and 48 h or after 5 d as indicator for the bacterial fermentation activity and compared. As a result, LABhe showed to be far less susceptible to high DM and osmolality respectively, compared to LABho. It was concluded that the in vitro test allows for qualitative assessment of the osmotolerance of LABhe, but not for quantitative ranking between LABhe strains.