# Energy-Rich Molecules and Group Transfer Potentials in Energetic Coupling Reactions

## TL;DR

The paper explains how certain molecules, like NTPs and thioesters, release energy through weak bonds and how this relates to biochemical energy coupling.

## Contribution

The paper clarifies the distinction between energy-rich molecules and group transfer potential, emphasizing their specific biochemical contexts.

## Key findings

- Energy-rich molecules like NTPs and thioesters release energy due to weak bonds being replaced by stronger ones.
- The term 'energy-rich' can be misleading and is best replaced by 'high group transfer potential' for certain molecules.
- Dioxygen does not fit the high group transfer potential concept, highlighting the need for distinct terminology.

## Abstract

The concept of energy-rich molecules is central to metabolic activity and the coupling of catabolic and anabolic processes. Here, we use the term “energy-rich” only in the (bio)chemical sense, i.e., for molecules containing particularly weak bonds that when exchanged for stronger bonds results in a release of energy (generally ≥ 20 kJ mol−1). The typical energy-rich molecules are nucleoside triphosphates (NTPs), thioesters, and dioxygen. It must be emphasized that the number of bonds is conserved in biochemical reactions, so that the difference in free energy between substrates and products only depends on the difference in bond energies. It is evident that using the term “energy-rich” for molecules with weak bonds is subject to misinterpretation. Therefore, some authors suggested to replace this term by molecule of high group transfer potential. This is justified for NTPs and thioesters, which have a high transfer potential for, respectively, phosphoryl or acyl groups, but not for dioxygen. Therefore, the concepts of energy-richness and group transfer potential should be treated as different and only be used within specific contexts. We discuss how these two notions can be used to understand the coupling mechanisms in biochemical processes as well as the interplay between thioesters, redox coupling, and phosphate transfer reactions.