# Effect of Hantavirus Infection on the Rodent Lung Microbiome: Specific Regulatory Roles of Host Species and Virus Types

## TL;DR

This study explores how Hantavirus infection affects the lung microbiome in different rodent species, revealing that host species and virus type influence microbial community changes.

## Contribution

The study identifies specific regulatory roles of host species and virus types in shaping the rodent lung microbiome during Hantavirus infection.

## Key findings

- HTNV-infected Apodemus agrarius showed increased evenness but decreased microbial richness.
- SEOV-infected Rattus norvegicus had no significant change in microbial richness compared to uninfected controls.
- LEfSe analysis identified taxa like Streptococcus and Chlamydia associated with infection status in specific host-virus pairings.

## Abstract

The lung-targeting characteristic of Hantavirus infection and the unclear mechanism underlying its interaction with the lung microbiome hampers the development of effective prevention and control strategies. In this study, lung tissues from Apodemus agrarius and Rattus norvegicus were collected at Hantavirus surveillance sites in Hunan Province. Metagenomic sequencing was subsequently applied to compare microbiome diversity, community structure, and function between infected and uninfected groups. Then the linear discriminant analysis effect size (LEfSe) was employed to identify key biomarkers. The results indicated that after infection with Hantaan virus (HTNV), Apodemus agrarius exhibited significantly increased evenness but markedly decreased richness of lung microbial communities, as reflected by consistent reductions in the number of observed species, Abundance-based Coverage Estimator (ACE) index, and Chao1 index. In contrast, Rattus norvegicus infected with Seoul virus (SEOV) showed no significant difference in microbial richness compared with uninfected controls, and even a slight increase was observed. These findings suggest that host species and virus type may play an important role in shaping microbial community responses. Furthermore, β-diversity analysis showed that the community structure was clearly separated by the host rodent species, as well as by their virus infection status. LEfSe analysis identified taxa with discriminatory power associated with infection status. Streptococcus agalactiae and Streptococcus were associated with SEOV-infected Rattus norvegicus, while Chlamydia and Chlamydia abortus were relatively enriched in uninfected Apodemus agrarius. This exploratory study reveals preliminary association between specific host—Hantavirus pairings (HTNV—Apodemus agrarius and SEOV—Rattus norvegicus) and the rodent lung microbiome, offering potential insights for future research into viral pathogenesis.