Transcriptomic Insights into Metabolic Reprogramming and Exopolysaccharide Synthesis in Porphyridium purpureum Under Gradual Nitrogen Deprivation
Maurean Guerreiro, Coline Emmanuel, Céline Dupuits, Christine Gardarin, Said Mouzeyar, João Varela, Jane Roche, Céline Laroche
TL;DR
This study explores how the red microalga Porphyridium purpureum responds to nitrogen deprivation, linking it to the production of exopolysaccharides and changes in metabolic pathways.
Contribution
The study provides new insights into the metabolic reprogramming and enzyme regulation in P. purpureum under nitrogen stress, particularly focusing on exopolysaccharide synthesis.
Findings
EPS release in P. purpureum coincides with reduced nitrate uptake, indicating a shift in carbon allocation.
Transcriptomic analysis reveals upregulation of CAZyme families GT4, GT8, and GT77 under nitrogen stress.
Nitrogen deprivation triggers phase-dependent metabolic changes, including stress-responsive and carbohydrate-related pathways.
Abstract
Porphyridium species are known red microalgae for producing valuable bioactive compounds such as sulfated exopolysaccharides (EPS) with diverse industrial biomedical applications due to their functional and rheological properties. Recent studies have investigated how abiotic stresses, particularly nitrogen deprivation, affect Porphyridium’s metabolic regulation and EPS production through transcriptomic analysis. Still, the mechanisms governing EPS biosynthesis and the involvement of carbohydrate-activated enzymes (CAZymes) remain poorly understood. This study investigated the progressive effects of nitrate consumption on the unicellular red alga, P. purpureum, by integrating physiological, biochemical, and transcriptomic analyses through RNA-Seq, further validated by RT-qPCR. P. purpureum displayed a gradual, phase-dependent metabolic response to progressive nitrogen stress. EPS release…
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Taxonomy
TopicsAlgal biology and biofuel production · Seaweed-derived Bioactive Compounds · Marine and coastal plant biology