# The Interference of Mnsod3 Enhances the Tolerance of Pleurotus ostreatus Mycelia to Abiotic Stress by Reshaping the Cell Wall

## TL;DR

This study shows that interfering with Mnsod3 in oyster mushrooms improves their ability to handle environmental stress by changing their cell walls.

## Contribution

The study reveals a novel role of Mnsod3 in regulating cell wall remodeling and abiotic stress tolerance in edible fungi.

## Key findings

- Mnsod3 interference increases transcript levels of cell wall synthase genes and tolerance to cell wall disruptors.
- RNAi of Mnsod3 reduces cell wall wrinkling under cold and light stress and restores cell wall smoothness.
- Mnsod3 plays a critical role in cell wall remodeling during abiotic stress in Pleurotus ostreatus.

## Abstract

In recent years, the response mechanism of Pleurotus ostreatus to abiotic stress has received widespread attention. MnSOD is an important antioxidant enzyme that has been widely studied in animals and plants because of its functions. However, there is little research on the function and regulatory mechanism of MnSOD in the growth and development of edible fungi. This study investigated the role of Mnsod3 in the growth and development of P. ostreatus. The results showed that during the nutritional growth stage, heat stress can cause the cell wall of mycelia to shrink and the cells to exhibit cytoplasmic wall separation. RNA-seq revealed that Mnsod3 interference is strongly correlated with increased transcript levels of cell wall synthase genes and with increased tolerance to cell wall disruptors. During the primordium formation stage, the mycelial cell wall also significantly wrinkled under cold and light stresses. RNAi of Mnsod3 alleviated the cell wall wrinkling caused by cold and light stress, restored the smoothness of the cell walls, and increased mycelial tolerance to abiotic stress. This may be related to the slower formation rate of primordia, but the specific molecular mechanism still needs further research. and slowed the rate of primordium formation. In summary, Mnsod3 plays an important role in the growth and development of P. ostreatus under abiotic stress and plays a critical regulatory role in cell wall remodeling under abiotic stress.