# Determining the Presence of a Polymorphism in the Calpastatin (CAST) Gene Locus and Its Influence on Some Fattening Traits in Danube White Pigs

## TL;DR

This study examines a genetic variation in the CAST gene of Danube White pigs and its effects on fattening traits like lean meat and back fat.

## Contribution

The study identifies a polymorphism in the CAST gene and links it to specific fattening traits in Danube White pigs.

## Key findings

- Two alleles (D and C) were identified at the CAST gene locus with frequencies of 60% and 40%, respectively.
- DD genotype pigs showed less back fat at X2, more back fat in LT, and higher lean meat percentage.
- Three genotypes (DD, CC, CD) were observed with frequencies of 40%, 21%, and 39%.

## Abstract

A total of 57 replacement gilts of the Danube White breed was used in a study carried out in the Agricultural Institute of Shumen. During the test period, for the trait “age at reaching 90 kg live weight” as well as the following traits, they were analyzed using a Piglog 105 device (portable ultrasound scanner, Frontmatec, Denmark): back fat thickness at points X1 and X2, growth intensity, back fat of m. Longissimus thoracis (LT), and lean meat percentage. DNA analysis was performed using the polymerase chain reaction restriction fragment-length polymorphism method (PCR-RFLP), with restriction endonuclease MspI. In the genotyped herd at the calpastatin gene locus, two alleles were identified with frequencies of 60% for allele D and 40% for allele C, and three genotypes DD, CC, and CD, with frequencies of 40%, 21%, and 39%, respectively. The percentage of animals with the DD genotype was the highest. They also had a lesser thickness of the back fat at point X2, a larger back fat of LT, and a higher percentage of lean meat.