SlACO1 and SlGARP Regulate Hormone-Mediated Metabolic Profiles in Tomato Fruit

TL;DR

This study shows how two genes in tomatoes affect fruit quality by influencing hormone levels and metabolite profiles.

Contribution

The study identifies SlACO1 and SlGARP as key regulators of hormone-mediated metabolic profiles in tomato fruit.

Findings

SlACO1 overexpression increases carotenoids, volatiles, and ethylene in tomato fruit.

SlGARP overexpression also boosts beneficial metabolites while reducing ABA levels.

RNAi knockdown of SlACO1 reduces color and key compounds like lycopene in fruit.

Abstract

Fruit quality is determined by multiple metabolites, which are mainly affected by plant hormones. In this study, two genes, ethylene-related gene SlACO1 and gibberellin-related gene SlGARP, were overexpressed (OE) and knocked down through RNAi in tomato, and the profiles of carotenoids, flavonoids, volatiles, and hormones were detected in the leaves and fruit. The color index significantly increased in SlACO1-OE fruit but significantly decreased in the SlACO1-RNAi line. Similarly, total carotenoids, volatiles, salicylic acid, and ethylene significantly increased in the fruits of SlACO1-OE and SlGARP-OE, whereas ABA decreased significantly. Some compounds, such as lycopene, 3-hexenal, and d-limonene, significantly increased in the fruit of SlACO1-OE but decreased in the SlACO1-RNAi line, indicating that SlACO1 might play an important role in the accumulation of these compounds. The functional characterization of SlACO1 and SlGARP would facilitate the improvement in tomato fruit quality.

Figures