Precision Profiling of the Cardiovascular Post-Translationally Modified Proteome

TL;DR

This paper reviews how post-translational modifications affect proteins in cardiovascular health and disease, using advanced proteomics techniques.

Contribution

The paper provides a comprehensive overview of proteoform analysis methods and their application to cardiovascular post-translational modifications.

Findings

Post-translational modifications significantly contribute to proteoform diversity in cardiovascular systems.

Mass spectrometry-based methods are effective for studying uncommon PTMs linked to cardiovascular disease.

PTMs are increasingly recognized as key players in cardiovascular physiological and pathological signaling.

Abstract

Proteins exist as multiple chemical and sequence-specific proteoforms, each of which may serve as a critical mediator of physiological or pathological signalling. This diversity arises from processes such as alternative splicing of gene transcripts, translation into amino acid sequences, and various post-translational modifications (PTMs), leading to an exponential increase in biological complexity. This manuscript provides an overview of the mechanisms underlying proteoform generation in biological systems and highlights strategies for their analysis using mass spectrometry (MS)-based proteomics and bioinformatics. Additionally, it focuses on recent findings linking PTMs to cardiovascular disease (CVD), highlighting the MS-based methods and workflows that have been used to study uncommon PTMs and their role in CVD. This review provides a comprehensive collection of tools and knowledge to explore the breadth of proteoforms, particularly PTMs, within their specific areas of interest in cardiovascular physiology.

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