Sex-Related Expression of Klotho in Rat Kidneys: Species Differences Between Rats and Mice
Davorka Breljak, Dean Karaica, Ivana Vrhovac Madunić, Vedran Micek, Tatjana Orct, Marija Ljubojević, Dubravka Rašić, Željka Vogrinc, Saša Kralik, Marko Gerić, Goran Gajski, Ivana Novak Jovanović, Lucia Nanić, Jasna Jurasović, Maja Peraica, Ivica Rubelj, Ivan Sabolić
TL;DR
This study explores how the anti-aging protein Klotho is expressed differently in rat kidneys based on sex and compares these findings to mice.
Contribution
The study reveals sex-related and species-specific differences in Klotho expression in rat and mouse kidneys.
Findings
rKl mRNA/protein is expressed in rat kidney cortex and outer stripe but not in the papilla.
Female rats show higher rKl expression, possibly due to androgen's inhibitory effects.
Sex-related differences in Klotho expression may influence electrolyte levels in urine.
Abstract
The anti-aging gene/protein Klotho (Kl), most present in kidneys, has been well studied in mice (mKl), but not in rats (rKl). This study investigated the renal rKl expression in male and female rats. Sex-related measurement of rKl-controlled electrolytes was performed in plasma/urine samples, as were tests on species differences in renal Kl expression (rats vs. mice). rKl mRNA/protein expression was studied by qRT-PCR/Western-blotting in renal total RNA/cell membranes and its localization by immunofluorescence microscopy. Urine/plasma ions (phosphate/total calcium) and macroelements (phosphorus/calcium) were measured biochemically and by ICP-MS, respectively. In rat kidneys, the rKl mRNA/protein was detected in the cortex, outer and inner stripe but not in the papilla, and was immunolocalized in the basolateral membrane of proximal tubules in the cortex and outer stripe, but not in the…
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Taxonomy
TopicsParathyroid Disorders and Treatments · Chronic Kidney Disease and Diabetes · Dialysis and Renal Disease Management