# Lysozyme Functionalized Alginate-Chitosan Beads and Films for Different Release Applications

## TL;DR

This paper describes the development of lysozyme-loaded beads and films for biomedical applications, showing high efficiency and antimicrobial properties.

## Contribution

The study introduces a novel biosystem using lysozyme-loaded alginate-chitosan beads and films with 100% encapsulation efficiency.

## Key findings

- Lysozyme-loaded beads showed about 99% anti-inflammatory activity.
- The developed biosystems demonstrated high antimicrobial activity against Micrococcus lysodeikticus.
- Lysozyme release was effectively controlled in buffer solutions mimicking cancer cell environments.

## Abstract

The main goal of this work was to develop nanoparticles of lysozyme (Lys) for biological and biomedical applications. The developed biosystems were based on Lys-loaded calcium alginate 2% and chitosan 1% beads and films with different concentrations of each polymer. Encapsulation efficiency was 100%. The ratio of adsorbed Lys on the films, Lys activity, and the release profile of Lys were measured using water and buffer solution at pH similar to the environment of cancer cells, at a controlled temperature of 37 °C and a constant speed, to assess the efficacy of the encapsulation process. Lys antimicrobial activity was assessed using Micrococcus lysodeikticus. Moreover, the anti-inflammatory and antioxidant properties of the developed biosystems were also evaluated. The anti-inflammatory activity of Lys released from calcium alginate 2%-chitosan 1% beads loaded with Lys was about 99%. These findings highlight the potential of the developed beads and films for biomedical applications, particularly in antimicrobial and anti-inflammatory therapies.